6-MALEIMIDOCAPROIC ACID SULFO-N-SUCCINIMIDYL ESTER

Chemical properties

White Solid

The Uses of 6-MALEIMIDOCAPROIC ACID SULFO-N-SUCCINIMIDYL ESTER

A water soluble sulfhydryl and amio reactive heterobifunctional crosslinking reagent. Spacer Arm: 9.4 Angstroms

The Uses of 6-MALEIMIDOCAPROIC ACID SULFO-N-SUCCINIMIDYL ESTER

A water soluble sulfhydryl and amio reactive heterobifunctional crosslinking reagent.Spacer Arm: 9.4 Angstroms

What are the applications of Application

6-Maleimidocaproic Acid Sulfo-N-Succinimidyl Ester is a water-soluble, heterobifunctional amine- and sulfhydryl-reactive crosslinker, 9.4 angstrom spacer arm

General Description

EMCS and its water-soluble analog Sulfo-EMCS are heterobifunctional cross-linkers that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7-9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. In aqueous solutions, hydrolytic degradation of the NHS ester is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group but will slowly hydrolyze and also lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugation experiments involving these cross-linkers are usually performed at pH 7.2-7.5, with the NHS-ester (amine-targeted) reaction being accomplished before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.