RIBOFLAVIN-5'-PHOSPHATE SODIUM SALT DIHYDRATE

Description

Riboflavin 5''-monophosphate (FMN) is a coenzyme that is tightly bound to enzymes catalyzing oxidation and reduction reactions in a variety of biosynthetic pathways. FMN also binds the FMN riboswitch (RFN element) on RNA to alter gene regulation. FMN is a substrate of FMN phosphohydrolases and is used to study their function.

Chemical properties

Yellowish crystalline powder

The Uses of RIBOFLAVIN-5'-PHOSPHATE SODIUM SALT DIHYDRATE

Riboflavin 5′-monophosphate (FMN) is used as a prosthetic group by oxidoreductase (one and two electron transfer) enzymes such as NADH dehydrogenases, nitric oxide synthases, and nitrilotriacetate monooxygenases. Riboflavin 5′-monophosphate is used as a substrate to study the specificity and kinetics of FMN phosphohydrolases. FMN is used to study molecular sensing based gene regulation by riboswitches.

The Uses of RIBOFLAVIN-5'-PHOSPHATE SODIUM SALT DIHYDRATE

Riboflavin 5''-(Dihydrogen Phosphate) Monosodium Salt Dihydrate is a phosphated nutritional factor found in milk, eggs, malted barley, liver, kidney, heart, leafy vegetables. Richest natural source is yeast. Minute amounts present in all plant and animal cells. Vitamin (enzyme cofactor).

What are the applications of Application

Riboflavin 5′-monophosphate sodium salt dihydrate is a phosphated enzyme cofactor (vitamin)

General Description

Riboflavin 5′-monophosphate (FMN) is highly soluble in water. Flavins are considered as blue-light absorbing dyes.

Biochem/physiol Actions

Riboflavin 5′-monophosphate (FMN) acts as a cofactor in flavoproteins and possess biological activity in enzymes?and photoreceptors.

Purification Methods

Purify FMN by paper chromatography using tert-butanol/water, cutting out the main spot and eluting it with water. It can also be purified by adsorption onto an apo-flavodoxin column, followed by elution and freeze drying. It crystallises from aqueous acidic solution. [Mayhew & Strating Eur J Biochem 59 539 1976, Beilstein 26 III/IV 2555.]