5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

Synonym(s):5-Bromo-4-chloro-3-indoxyl phosphate p-toluidine salt;BCIP p-toluidine salt;X-phosphate p-toluidine salt

CAS NO.：6578-06-9
Empirical Formula: C8H6BrClNO4P.C7H9N
Molecular Weight: 433.63
MDL number: MFCD00040636
EINECS: 229-506-1
SAFETY DATA SHEET (SDS)
Update Date: 2025-10-29 15:43:00

5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt Structural

What is 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt?

Chemical properties

White to light brown powder

The Uses of 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.

What are the applications of Application

BCIP p-toluidine salt is a histochemical substrate for alkaline phosphatase

General Description

BCIP serves as substrate for alkaline phosphatase. The reaction product has a blue color and is insoluble in water. The blue color can be visually detected.

Biochem/physiol Actions

5-bromo-4-chloro-3-indolyl phosphate (BCIP) can be used to visualize the phosphatase activity. It can be used as a substrate to monitor secreted embryonic alkaline phosphatase?activity in solution.

Properties of 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

Melting point:	194-195℃
storage temp.	-20°C
solubility	DMF: 20 mg/mL
form	tablets
color	White to Orange to Green
CAS DataBase Reference	6578-06-9(CAS DataBase Reference)
EPA Substance Registry System	1H-Indol-3-ol, 5-bromo-4-chloro-, dihydrogen phosphate (ester), compd. with 4-methylbenzenamine (1:1) (6578-06-9)

Safety information for 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

Signal word	Danger
Pictogram(s)	Flame Flammables GHS02 Exclamation Mark Irritant GHS07 Health Hazard GHS08
GHS Hazard Statements	H226:Flammable liquids H319:Serious eye damage/eye irritation
Precautionary Statement Codes	P201:Obtain special instructions before use. P210:Keep away from heat/sparks/open flames/hot surfaces. — No smoking. P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P280:Wear protective gloves/protective clothing/eye protection/face protection. P308+P313:IF exposed or concerned: Get medical advice/attention. P370+P378:In case of fire: Use … for extinction.