5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
Synonym(s):5-Bromo-4-chloro-3-indoxyl phosphate p-toluidine salt;BCIP p-toluidine salt;X-phosphate p-toluidine salt
- CAS NO.：6578-06-9
- Empirical Formula: C8H6BrClNO4P.C7H9N
- Molecular Weight: 433.63
- MDL number: MFCD00040636
- EINECS: 229-506-1
- SAFETY DATA SHEET (SDS)
- Update Date: 2026-01-13 11:20:08
What is 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt?
Chemical properties
White to light brown powder
The Uses of 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
What are the applications of Application
BCIP p-toluidine salt is a histochemical substrate for alkaline phosphatase
General Description
BCIP serves as substrate for alkaline phosphatase. The reaction product has a blue color and is insoluble in water. The blue color can be visually detected.
Biochem/physiol Actions
5-bromo-4-chloro-3-indolyl phosphate (BCIP) can be used to visualize the phosphatase activity. It can be used as a substrate to monitor secreted embryonic alkaline phosphatase?activity in solution.
Properties of 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
| Melting point: | 194-195℃ |
| storage temp. | -20°C |
| solubility | DMF: 20 mg/mL |
| form | tablets |
| color | White to Orange to Green |
| CAS DataBase Reference | 6578-06-9(CAS DataBase Reference) |
| EPA Substance Registry System | 1H-Indol-3-ol, 5-bromo-4-chloro-, dihydrogen phosphate (ester), compd. with 4-methylbenzenamine (1:1) (6578-06-9) |
Safety information for 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
| Signal word | Danger |
| Pictogram(s) |
 Flame Flammables GHS02  Exclamation Mark Irritant GHS07  Health Hazard GHS08 |
| GHS Hazard Statements |
H226:Flammable liquids H319:Serious eye damage/eye irritation |
| Precautionary Statement Codes |
P201:Obtain special instructions before use. P210:Keep away from heat/sparks/open flames/hot surfaces. — No smoking. P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P280:Wear protective gloves/protective clothing/eye protection/face protection. P308+P313:IF exposed or concerned: Get medical advice/attention. P370+P378:In case of fire: Use … for extinction. |
Computed Descriptors for 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
| InChIKey | QEIFSLUFHRCVQL-UHFFFAOYSA-N |
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt manufacturer
GVSK Pharma Private Limited
Siri Organics
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