3,3',4,4'-Biphenyltetramine tetrahydrochloride

Chemical properties

Grey Solid

The Uses of 3,3',4,4'-Biphenyltetramine tetrahydrochloride

A substrate for peroxidase, and a reagent for the spectrophotometric determination of selenium

The Uses of 3,3',4,4'-Biphenyltetramine tetrahydrochloride

3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride has been used:

• in the detection of hydrogen peroxide in rice leaves
• as a substrate for rabbit peroxidase in immunohistochemistry of mice brain sections
• in immunocytochemistry of muscle-derived stem cells (MDSCs)

The Uses of 3,3',4,4'-Biphenyltetramine tetrahydrochloride

3,3′-Diaminobenzidine tetrahydrochloride has been used for immunohistochemistry.

What are the applications of Application

DAB, 50X is a product used in HRP-based assays suitable for immunoblotting and immunohistochemical staining of nucleic acids and proteins

General Description

3,3′-Diaminobenzidine (DAB) is a chromogenic substrate for peroxidase enzyme. It is converted to an insoluble precipitate after oxidation by peroxidase.

Biochem/physiol Actions

3,3′-Diaminobenzidine (DAB) is a chromogenic substrate for peroxidase enzyme. It is converted to an insoluble precipitate after oxidation by peroxidase. This feature is exploited for visualization in histochemical (HC) and immunohistochemical (IHC) staining.

Safety Profile

Suspected carcinogen withexperimental neoplastigenic and tumorigenic data. Poisonby intraperitoneal route. When heated to decomposition itemits very toxic fumes of HCl and NOx.

Purification Methods

Dissolve the salt in water and precipitate it by adding conc HCl, then drying it over solid NaOH. [Beilstein 13 IV 530.]