3,3',5,5'-Tetramethylbenzidine dihydrochloride
Synonym(s):4,4′-Diamino-3,3′,5,5′-tetramethylbiphenyl dihydrochloride
- CAS NO.：64285-73-0
- Empirical Formula: C16H21ClN2
- Molecular Weight: 276.81
- MDL number: MFCD00012961
- EINECS: 264-769-6
- SAFETY DATA SHEET (SDS)
- Update Date: 2026-04-22 18:57:36
What is 3,3',5,5'-Tetramethylbenzidine dihydrochloride?
Chemical properties
3,3',5,5'-Tetramethylbenzidine dihydrochloride is white powder
The Uses of 3,3',5,5'-Tetramethylbenzidine dihydrochloride
3,3',5,5'-Tetramethylbenzidine dihydrochloride is a peroxidase substrate suitable for use in ELISA procedures.
The Uses of 3,3',5,5'-Tetramethylbenzidine dihydrochloride
3,3′,5,5′-Tetramethylbenzidine dihydrochloride has been used as a substrate for the
- anti-mouse IgG (? specific) horseradish peroxidase-conjugated antibody in the ELISA of mice sera
- anti-mouse peroxidase antibody in the protein tyrosine phosphatase assay of naive and effector T cell lysate
- in enzymatic hydroperoxide (EH) assay and in prooxidants–antioxidants balance (PAB) assay of serum samples from diabetic patients
What are the applications of Application
3,3′,5,5′-Tetramethylbenzidine dihydrochloride anhydrous is a peroxidase substrate suitable for use in ELISA procedures
Properties of 3,3',5,5'-Tetramethylbenzidine dihydrochloride
| Melting point: | ≥300 °C |
| Flash point: | 54 °C |
| storage temp. | 2-8°C |
| form | tablet |
| color | White to off-white |
| Sensitive | Moisture & Light Sensitive |
| InChI | InChI=1S/C16H20N2.ClH/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;/h5-8H,17-18H2,1-4H3;1H |
| CAS DataBase Reference | 64285-73-0(CAS DataBase Reference) |
| EPA Substance Registry System | [1,1'-Biphenyl]-4,4'-diamine, 3,3',5,5'-tetramethyl-, dihydrochloride (64285-73-0) |
Safety information for 3,3',5,5'-Tetramethylbenzidine dihydrochloride
| Signal word | Warning |
| Pictogram(s) |
 Exclamation Mark Irritant GHS07 |
| GHS Hazard Statements |
H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation |
| Precautionary Statement Codes |
P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P304+P340:IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. P405:Store locked up. |
Computed Descriptors for 3,3',5,5'-Tetramethylbenzidine dihydrochloride
| InChIKey | OBWSOTREAMFOCQ-UHFFFAOYSA-N |
| SMILES | CC1=C(C(C)=CC(C2C=C(C)C(N)=C(C)C=2)=C1)N.Cl |
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