Triphenylformazan

Synonym(s):TTC formazan

CAS NO.：531-52-2
Empirical Formula: C19H16N4
Molecular Weight: 300.36
MDL number: MFCD00020647
EINECS: 208-509-1
SAFETY DATA SHEET (SDS)
Update Date: 2025-03-15 17:35:32

What is Triphenylformazan?

Chemical properties

Dark brown crystalline powder

The Uses of Triphenylformazan

Germination and viability tests.

The Uses of Triphenylformazan

1,3,5-Triphenyltetrazolium formazan has been used:

for the quantification of 2,3,5-triphenyltetrazolium chloride (TTC) reduction activity in bacterial cells
as a standard to study the optical density of formazan produced by TTC-treated milled seeds
to stain heart tissue for infarct size assessment or undergo perfusion fixation to allow morphometric studies in addition to histology and immunohistochemistry

General Description

1,3,5-Triphenyltetrazolium formazan is an insoluble compound produced upon the reduction of 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) salt, which is an indicator of cell redox potential. This reaction is irreversible. TTC formazan is brightly colored and can be quantified using a spectrophotometer.

Properties of Triphenylformazan

Melting point:	169-170 °C
Boiling point:	431.59°C (rough estimate)
Density	1.1454 (rough estimate)
refractive index	1.6140 (estimate)
storage temp.	Store at 2-8
solubility	dioxane: 50 mg/mL, clear
form	powder to crystaline
pka	11.56±0.10(Predicted)
color	Orange to Brown to Dark red
BRN	753057
CAS DataBase Reference	531-52-2(CAS DataBase Reference)
NIST Chemistry Reference	Triphenylformazan(531-52-2)
EPA Substance Registry System	Diazene, phenyl[phenyl(phenylhydrazono)methyl]- (531-52-2)

Safety information for Triphenylformazan

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07 Environment GHS09
GHS Hazard Statements	H302:Acute toxicity,oral H400:Hazardous to the aquatic environment, acute hazard
Precautionary Statement Codes	P273:Avoid release to the environment.