Triphenylformazan
Synonym(s):TTC formazan
- CAS NO.：531-52-2
- Empirical Formula: C19H16N4
- Molecular Weight: 300.36
- MDL number: MFCD00020647
- EINECS: 208-509-1
- SAFETY DATA SHEET (SDS)
- Update Date: 2026-05-28 02:50:42
What is Triphenylformazan?
Chemical properties
Dark brown crystalline powder
The Uses of Triphenylformazan
Germination and viability tests.
The Uses of Triphenylformazan
1,3,5-Triphenyltetrazolium formazan has been used:
- for the quantification of 2,3,5-triphenyltetrazolium chloride (TTC) reduction activity in bacterial cells
- as a standard to study the optical density of formazan produced by TTC-treated milled seeds
- to stain heart tissue for infarct size assessment or undergo perfusion fixation to allow morphometric studies in addition to histology and immunohistochemistry
General Description
1,3,5-Triphenyltetrazolium formazan is an insoluble compound produced upon the reduction of 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) salt, which is an indicator of cell redox potential. This reaction is irreversible. TTC formazan is brightly colored and can be quantified using a spectrophotometer.
Properties of Triphenylformazan
| Melting point: | 169-170 °C |
| Boiling point: | 431.59°C (rough estimate) |
| Density | 1.1454 (rough estimate) |
| refractive index | 1.6140 (estimate) |
| storage temp. | Store at 2-8 |
| solubility | dioxane: 50 mg/mL, clear |
| form | powder to crystaline |
| pka | 11.56±0.10(Predicted) |
| color | Orange to Brown to Dark red |
| BRN | 753057 |
| CAS DataBase Reference | 531-52-2(CAS DataBase Reference) |
| NIST Chemistry Reference | Triphenylformazan(531-52-2) |
| EPA Substance Registry System | Diazene, phenyl[phenyl(phenylhydrazono)methyl]- (531-52-2) |
Safety information for Triphenylformazan
| Signal word | Warning |
| Pictogram(s) |
 Exclamation Mark Irritant GHS07  Environment GHS09 |
| GHS Hazard Statements |
H302:Acute toxicity,oral H400:Hazardous to the aquatic environment, acute hazard |
| Precautionary Statement Codes |
P273:Avoid release to the environment. |
Computed Descriptors for Triphenylformazan
Triphenylformazan manufacturer
Innovative
Mumbai
product: 1,3,5-Triphenyltetrazolium Formazan (TTC Formazan) (CAS Number: 531-52-2)
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