ORCEIN

Synonym(s):Natural Red 28

CAS NO.：1400-62-0
Empirical Formula: C28H24N2O7
Molecular Weight: 500.49936
MDL number: MFCD00062310
EINECS: 215-750-6
Update Date: 2025-05-13 21:09:50

What is ORCEIN?

Chemical properties

dark brown crystals or powder

The Uses of ORCEIN

Orcein (cas# 1400-62-0) is an organic dye and histochemical stain. Orcein has also been used as a stain for extracellular matrices. Dyes and metabolites.

The Uses of ORCEIN

Biological stain.

What are the applications of Application

Orcein (Synthetic) is a useful microscopy dye for flagella stains

Definition

ChEBI: A member of the class of benzofurans that is dibenzo[b,d]furan-3,7-dione bearing two methyl substituents at positions 1 and 9 as well as two 2,4-dihydroxy-6-methylanilino substituents at positions 2 and 8.

General Description

Orcein is a weakly acidic orange-red fluorochrome and is mainly required for the staining of elastic tissue. It is not specific for chromatin staining, however, in acidic solutions it works as a basic dye and stains basophilic chromosomes purple red.

Properties of ORCEIN

Boiling point:	591.36°C (rough estimate)
Density	1.3224 (rough estimate)
refractive index	1.5800 (estimate)
storage temp.	room temp
solubility	Insoluble in water, benzene, chloroform, ether; soluble in ethanol, acetone, acetic acid; soluble in aqueous alkali
pka	4.0, 6.9, 13.4(at 25℃)
form	Powder
color	Dark brown to brown-black
Water Solubility	Soluble in acetone, alcohol, acetic acid (red color), and dilute aqueous alkali (blue-violet color). Insoluble in water.
λmax	575 nm
Merck	14,6863
Stability:	Stable.
Biological Applications	Diagnosis of liver biopsy; detecting Candida,hepatitis B surface antigen in fixed tissues,histamines,HBsAg in liver cell,microorganisms
EPA Substance Registry System	Orcein (1400-62-0)

Safety information for ORCEIN

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H302:Acute toxicity,oral H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation
Precautionary Statement Codes	P264:Wash hands thoroughly after handling. P264:Wash skin thouroughly after handling. P270:Do not eat, drink or smoke when using this product. P280:Wear protective gloves/protective clothing/eye protection/face protection. P330:Rinse mouth.