N-Benzoyl-DL-arginine-4-nitroanilide hydrochloride
Synonym(s):Nα-Benzoyl-DL -arginine p-nitroanilide hydrochloride;BANI;BAPNA
- CAS NO.：911-77-3
- Empirical Formula: C19H23ClN6O4
- Molecular Weight: 434.88
- MDL number: MFCD00012846
- EINECS: 213-011-2
- SAFETY DATA SHEET (SDS)
- Update Date: 2026-05-27 23:05:18
What is N-Benzoyl-DL-arginine-4-nitroanilide hydrochloride?
Chemical properties
Light yellow fine crystalline powder
The Uses of N-Benzoyl-DL-arginine-4-nitroanilide hydrochloride
Substrate for trypsin, papain, ficin, bromelain, plasmin, and cathepsin B.
The Uses of N-Benzoyl-DL-arginine-4-nitroanilide hydrochloride
Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride has been used as a substrate for trypsin and elastase in enzyme analysis.
What are the applications of Application
N-α-Benzoyl-DL-arginine 4-nitroanilide monohydrochloride is a pNA-based chromogenic trypsin substrate
General Description
Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride (DL-BAPNA) is a chromogenic substrate for proteolytic enzymes such as trypsin, amidase, and balterobin. Hydrolysis of DL-BAPNA at the bond between the arginine and the p-nitroaniline moieties releases the chromophore p-nitroaniline, which can be detected by colorimetric analysis.
Properties of N-Benzoyl-DL-arginine-4-nitroanilide hydrochloride
| Melting point: | ~275 °C (dec.) |
| storage temp. | -20°C |
| solubility | DMF: 1 mg/ml; DMSO: 10 mg/ml; DMSO:PBS (pH 7.2) (1:8): 0.11 mg/ml |
| form | Powder |
| color | White to off-white |
| BRN | 4081878 |
| CAS DataBase Reference | 911-77-3(CAS DataBase Reference) |
| EPA Substance Registry System | Benzamide, N-[4-[(aminoiminomethyl)amino]-1-[[(4-nitrophenyl)amino]carbonyl]butyl]-, monohydrochloride (911-77-3) |
Safety information for N-Benzoyl-DL-arginine-4-nitroanilide hydrochloride
| Signal word | Warning |
| Pictogram(s) |
 Exclamation Mark Irritant GHS07 |
| GHS Hazard Statements |
H317:Sensitisation, Skin H319:Serious eye damage/eye irritation |
| Precautionary Statement Codes |
P280:Wear protective gloves/protective clothing/eye protection/face protection. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. |
Computed Descriptors for N-Benzoyl-DL-arginine-4-nitroanilide hydrochloride
| InChIKey | DEOKFPFLXFNAON-UHFFFAOYSA-N |
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