D-Biotin p-nitrophenyl ester

Synonym(s):BNP;Biotin-ONp;NPPB;d-Biotin p-nitrophenyl ester;4-Nitrophenyl (+)-biotinate

CAS NO.：33755-53-2
Empirical Formula: C16H19N3O5S
Molecular Weight: 365.4
MDL number: MFCD15141972
EINECS: 1533716-785-6
SAFETY DATA SHEET (SDS)
Update Date: 2026-09-12 18:48:34

What is D-Biotin p-nitrophenyl ester?

Chemical properties

White to off-white powder

The Uses of D-Biotin p-nitrophenyl ester

(+)-Biotin 4-nitrophenyl ester may be used in the preparation of biotin tagged ARC (adenosine-oligoarginine conjugate) derivative during cellular protein uptake studies. It may also be used to prepare biotinylated quinazoline derivatives, which shows inhibitory activities against VEGFR-2 (vascular endothelial growth factor receptor-2) tyrosine kinase. (+)-Biotin 4-nitrophenyl ester undergoes hydrolysis in the presence of Na[¹⁷O]H to form [¹⁷O₂]biotin, which can be used in solid-state ¹⁷O NMR spectroscopic experiments to analyze large protein-ligand complexes.

The Uses of D-Biotin p-nitrophenyl ester

For research use only. Not for use in diagnostic procedures.
Brain natriuretic peptide EIA Kit has been used to measure brain natriuretic peptide (BNP) secreted in human neonatal ventricular cardiomyocyte culture.

General Description

The BNP Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting BNP peptide based on the principle of Competitive Enzyme Immunoassay.

Biochem/physiol Actions

Brain natriuretic peptide (BNP) expressed in mammalian brain and atrial natriuretic peptide (ANP) facilitates various physiological functions including regulation of blood pressure and cardiac remodeling. BNP acts as a potential biomarker of high left ventricular end-diastolic pressure in patients with symptomatic left ventricular (LV) dysfunction. Elevated expression of BNP is observed in acute myocardial infarction (AMI) patients. BNP is also implicated in several biological functions such as diuresis, natriureis, hypotensive action and inhibition of aldosterone secretion.

Purification Methods

The ester has been recrystallised by dissolving 2g in 95% EtOH (30mL), heated to dissolve, then cooled in an ice-water bath. The crystals are collected, washed with ice-cold 95% EtOH (5mL) and dried over P2O5. The RF on silica plates (CHCl3/MeOH 19:1) is 0.19 [Bodanszky & Fagan J Am Chem Soc 99 235 1977].

Properties of D-Biotin p-nitrophenyl ester

Melting point:	163-165 °C(lit.)
Boiling point:	642.9±50.0 °C(Predicted)
Density	1.326±0.06 g/cm3(Predicted)
storage temp.	Sealed in dry,Room Temperature
solubility	methanol: soluble25mg/mL, clear, colorless to greenish-yellow
form	Solid
pka	13.89±0.40(Predicted)
color	White to Off-White
optical activity	[α]25/D +51°, c = 2 in DMF: AcOH (99:1)
BRN	5795805
CAS DataBase Reference	33755-53-2

Safety information for D-Biotin p-nitrophenyl ester

Signal word	Warning
Pictogram(s)	Corrosion Corrosives GHS05
GHS Hazard Statements	H290:Corrosive to Metals
Precautionary Statement Codes	P234:Keep only in original container. P390:Absorb spillage to prevent material damage.