4-NITROPHENYL ACETATE

Synonym(s):Acetic acid 4-nitrophenyl ester

CAS NO.：830-03-5
Empirical Formula: C8H7NO4
Molecular Weight: 181.15
MDL number: MFCD00007326
EINECS: 212-593-5
SAFETY DATA SHEET (SDS)
Update Date: 2026-04-24 14:58:25

What is 4-NITROPHENYL ACETATE?

Chemical properties

off-white or beige powder

The Uses of 4-NITROPHENYL ACETATE

4-Nitrophenyl Acetate have been used as a substrate to study for the enzymatic activity of Carbonic Anhydrase III isolated from bovine skeletal muscle.

The Uses of 4-NITROPHENYL ACETATE

4-Nitrophenyl acetate is used with iodoacetic acid for reductive cleavage of methionine-containing peptides. It is also used as a substrate that has been used in assays for esterase and lipase activity. Inorganic complexes have been evaluated for their methanolysis or hydrolysis activity using p-nitrophenyl acetate.

What are the applications of Application

4-Nitrophenyl acetate is a chromogenic esterase substrate

Definition

ChEBI: An acetate ester resulting from the formal condensation of the hydroxy group of 4-nitrophenol with the carboxy group of acetic acid.

Synthesis Reference(s)

Synthesis, p. 991, 1981 DOI: 10.1055/s-1981-29674

Safety Profile

Poison by intravenous route. Whenheated to decomposition it emits toxic fumes of NOx.

Purification Methods

Recrystallise the ester from absolute EtOH [Moss et al. J Am Chem Soc 108 5520 1986]. [Beilstein 6 IV 1298.]

Properties of 4-NITROPHENYL ACETATE

Melting point:	75-77 °C (lit.)
Boiling point:	314.24°C (rough estimate)
Density	1.4283 (rough estimate)
vapor pressure	1.28Pa at 20℃
refractive index	1.5468 (estimate)
storage temp.	-20°C
solubility	0.53g/l
form	Powder
color	Green
Water Solubility	Insoluble in water.
BRN	515874
Stability:	Stable. Combustible. Incompatible with strong oxidizing agents.
CAS DataBase Reference	830-03-5(CAS DataBase Reference)
EPA Substance Registry System	Acetic acid, 4-nitrophenyl ester (830-03-5)

Safety information for 4-NITROPHENYL ACETATE

Signal word	Danger
Pictogram(s)	Flame Over Circle Oxidizers GHS03 Corrosion Corrosives GHS05 Exclamation Mark Irritant GHS07
GHS Hazard Statements	H272:Oxidising liquids;Oxidising solids H317:Sensitisation, Skin H318:Serious eye damage/eye irritation
Precautionary Statement Codes	P210:Keep away from heat/sparks/open flames/hot surfaces. — No smoking. P220:Keep/Store away from clothing/…/combustible materials. P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P280:Wear protective gloves/protective clothing/eye protection/face protection. P302+P352:IF ON SKIN: wash with plenty of soap and water. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.