BAPTA

Synonym(s):2,2′-(Ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid;BAPTA;Ethylenedioxybis(o-phenylenenitrilo)tetraacetic acid;Ethylenedioxybis(o-phenylenenitrilo)tetraacetic acid

CAS NO.：85233-19-8
Empirical Formula: C22H24N2O10
Molecular Weight: 476.43
MDL number: MFCD00036255
SAFETY DATA SHEET (SDS)
Update Date: 2026-03-13 23:13:44

What is BAPTA?

Description

BAPTA free acid (85233-19-8) is a selective extracellular Ca2+ chelator. It is a widely used calcium sponge for chelating Ca2+?outside of intact cells.1 It’s a useful tool to probe the involvement of extracellular Ca2+ in cellular signaling.2,3 Non-cell-permeable.

Chemical properties

White Solid

The Uses of BAPTA

Highly selective calcium chelating reagent. Suitable for the spectrophotometric monitoring of Ca2+ levels.

The Uses of BAPTA

As calcium chelator; 19F labelled forms for measurement of intracellular calcium.

The Uses of BAPTA

BAPTA is a highly selective calcium chelating reagent. Lipophilic diesters of the chelating agent 1,2-Bis(2 aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA) are used for inhibition of proteolytic activities of certain metalloproteinases, calpain and TACE.

Definition

ChEBI: A polyamino carboxylic acid in which bis(carboxymethyl)nitrilo groups are bonded to C-2 and C-2' of 1,1'-[ethane-1,2-diylbis(oxy)]dibenzene.

What are the applications of Application

BAPTA, Free Acid is a selective calcium chelator with 105-fold greater affinity for Ca2+ than Mg2+

General Description

In nature 1,2-Bis(2-Aminophenoxy)ethane-N,N,N′,N′-tetra acetic acid (BAPTA) is a main metabolic product of BAPTA -AM (BAPTA-tetra(actoxymethyl ester), a neuro protective agent in cerebral ischemia, in rats.BAPTA is an intercellular Ca²⁺ chelator.

Biological Activity

Selective calcium chelator.

Storage

Store at +4°C

References

1) Munoz et al. (2015), Different Stress-Induced Calcium Signatures Are Reported by Aequorin-Mediated Calcium Measurements in Living Cells of Aspergillus fumigatus; PLoS One, 10(9) e0138008 2) Wozniak et al. (2017), Extracellular Ca2+ is Required for Fertilization in the African Clawed Frog, Xenopus laevis; PLoS One, 12(1) e0170405 3) Didiasova et al. (2015), STIM1/ORAI1-mediated Ca2+ Influx Regulates Enolase-1 Exteriorization; J. Biol. Chem., 290 11983

Properties of BAPTA

Melting point:	177-179 °C
Boiling point:	575.89°C (rough estimate)
Density	1.4482 (rough estimate)
RTECS	MC0423000
refractive index	1.6500 (estimate)
storage temp.	2-8°C
solubility	Soluble in dimethyl sulfoxide
pka	1.40±0.10(Predicted)
form	Powder
color	White to off-white
Water Solubility	Soluble in water and sodium bicarbonate.
λmax	209 nm, 254 nm, 203 nm, 274 nm
Merck	14,957
BRN	5192406
Stability:	Stable for 1 year from date of purchase as supplied. Solutions in DMSO may be stored at -20°C for up to 1 month.
Biological Applications	Calcium indicators; zinc indicators; treating acute cell death,cerebral infarction,myocardial infarction,hepatonecrosis,kidney ischemic necrosis,necrotizing pancreatitis,amyloidosis,atherosclerosis,diseases characteriz
CAS DataBase Reference	85233-19-8(CAS DataBase Reference)

Safety information for BAPTA

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes	P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P264:Wash hands thoroughly after handling. P264:Wash skin thouroughly after handling. P280:Wear protective gloves/protective clothing/eye protection/face protection. P304+P340:IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. P405:Store locked up.