Ac-YVAD-AFC

Description

Ac-YVAD-AFC is a substrate whose amino acids YVAD have been shown to be a preferred cleavage site for caspase-1 and -4. Caspase activity can be quantified by fluorescent detection of free AFC (also known as 7-amino-4-trifluoromethylcoumarin), which is excited at 400 nm and emits at 505 nm.

The Uses of Ac-YVAD-AFC

N-Acetyl-Tyr-Val-Ala-Asp-7-amido-4-trifluoromethylcoumarin is a substrate whose amino acids are shown as a preferred cleavage site for Caspase-1 and 4. Useful for caspase research.

What are the applications of Application

Ac-YVAD-AFC is a fluorogenic substrate for caspase-1 and caspase-4

Definition

ChEBI: Ac-Tyr-Val-Ala-Asp-7-amino-4-trifluoromethylcoumarin is a tetrapeptide obtained by formal condensation of the C-terminal carboxy group of Ac-Tyr-Val-Ala-Asp with the amino group of 7-amino-4-trifluoromethylcoumarin. It has a role as a protease inhibitor. It is a tetrapeptide, a member of acetamides, a member of coumarins and an organofluorine compound. It is functionally related to a 7-amino-4-(trifluoromethyl)coumarin.

Biological Activity

Fluorogenic peptide substrate for caspase-1 (ICE).