5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN

Synonym(s):5-Carboxy-di-O-acetylfluorescein N-succinimidyl ester;CFSE

CAS NO.：150347-59-4
Empirical Formula: C29H19NO11
Molecular Weight: 557.46
MDL number: MFCD31812843
SAFETY DATA SHEET (SDS)
Update Date: 2026-08-27 11:54:19

5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN Structural

What is 5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN?

Chemical properties

5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN is Off-white to light yellow powder

The Uses of 5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN

Amine-reactive succinimidyl ester can potentially be used for long-term pH studies of live cells, producing a conjugate with the pH-sensitive properties of carboxyfluorescein.

The Uses of 5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN

A 5(6)-Carboxyfluorescein (C178215) derivative used as a flurorescent probe. 5(6)-Carboxyfluorescein 3’,6’-Diacetate N-Succinimidyl Ester is used to monitor lymphocyte proliferation by covalently labelling long-lived intracellular molecules with fluorescent dye.

The Uses of 5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN

5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN is a Non-fluorescent cell permeant amine-reactive probe for long term tracing of cell.

What are the applications of Application

CFSE is a cell permeable amine-reactive fluorescent label

General Description

5-(and-6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is a fluorescent dye, which is membrane permeable. It labels cells by covalently binding to intracellular molecules. CFSE is used to track lymphocyte migration and proliferation.

Biochem/physiol Actions

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester is used as an indicator for esterase activity.

Properties of 5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN

Melting point:	152-154 °C(lit.)
storage temp.	-20°C
solubility	DMF: soluble
form	Solid
pka	6.45(at 25℃)
color	Off-white to yellow
PH Range	Weak green ' uorescence (6.0) to strong green ' uorescence (7.2)
λmax	<300nm
Major Application	Diagnosis of cancer, restricting HIV replication, antiinflammatory agent, detecting bacteria, cell proliferation assays

Safety information for 5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H302:Acute toxicity,oral H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes	P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P280:Wear protective gloves/protective clothing/eye protection/face protection. P301+P312:IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell. P302+P352:IF ON SKIN: wash with plenty of soap and water. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.