5-Bromo-4-chloro-3-indolyl-beta-D-galactoside

Synonym(s):X-Gal;BCIG;bromo-4-chloro-3-indolyl-β-d-galactopyranoside, 5-;5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside;5-Bromo-4-chloro-3-indolyl β-D-galactoside

CAS NO.：7240-90-6
Empirical Formula: C14H15BrClNO6
Molecular Weight: 408.63
MDL number: MFCD00005666
EINECS: 230-640-8
SAFETY DATA SHEET (SDS)
Update Date: 2025-12-07 19:09:50

5-Bromo-4-chloro-3-indolyl-beta-D-galactoside Structural

What is 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside?

Chemical properties

White Powder

The Uses of 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside

A substrate for -Galactosidase that is converted by the enzyme into an intense indigo-blue chromophore (615 nm)

The Uses of 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside

A substrate for β-Galactosidase that is converted by the enzyme into an intense indigo-blue chromophore (615 nm).

The Uses of 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside

magenta b-galactosidase substrate

The Uses of 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside

In histochemical staining, in blue-white selection of bacterial colonies with lacZ gene activity during cloning experiments.

What are the applications of Application

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside is a chromogenic histochemical substrate for β-galactosidase

Definition

ChEBI: An indolyl carbohydrate that is the beta-D-galactoside of 3-hydroxy-1H-indole in which the indole moiety is substituted at positions 4 and 5 by chlorine and bromine, respectively. It is used to test for he presence of an enzyme, beta-galactosidase, which cleaved the glycosidic bond to give 5-bromo-4-chloro-3-hydroxy-1H-indole, which immediately dimerises to give an intensely blue product.

Storage

+4°C

Properties of 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside

Melting point:	230 °C
Boiling point:	673.9±55.0 °C(Predicted)
alpha	-64 º (C=1, H2O/DMF (1/1))
Density	1.5563 (rough estimate)
refractive index	1.6110 (estimate)
storage temp.	-20°C
solubility	Soluble in 100 mM in DMSO.
form	Powder
pka	12.74±0.70(Predicted)
color	White
Odor	Odorless
Sensitive	Moisture & Light Sensitive
Merck	14,10074
BRN	1402009
Stability:	Stable. Incompatible with strong oxidizing agents.
CAS DataBase Reference	7240-90-6(CAS DataBase Reference)

Safety information for 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
Precautionary Statement Codes	P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P270:Do not eat, drink or smoke when using this product. P271:Use only outdoors or in a well-ventilated area. P280:Wear protective gloves/protective clothing/eye protection/face protection. P312:Call a POISON CENTER or doctor/physician if you feel unwell. P330:Rinse mouth. P363:Wash contaminated clothing before reuse. P302+P352:IF ON SKIN: wash with plenty of soap and water. P304+P340:IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing. P403:Store in a well-ventilated place.