5-BROMO-4-CHLORO-3-INDOLYL B-D-
Synonym(s):X-GlcA;X-glucuronide CHA salt
- CAS NO.：114162-64-0
- Empirical Formula: C20H26BrClN2O7
- Molecular Weight: 521.79
- MDL number: MFCD13185253
- EINECS: 601-302-0
- SAFETY DATA SHEET (SDS)
- Update Date: 2026-05-11 15:21:01
What is 5-BROMO-4-CHLORO-3-INDOLYL B-D-?
Chemical properties
White Solid
The Uses of 5-BROMO-4-CHLORO-3-INDOLYL B-D-
5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt has been used as a histochemical reagent in X-GLUC staining.
The Uses of 5-BROMO-4-CHLORO-3-INDOLYL B-D-
Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype.
X-GlcA has been used in histochemical staining of root sections for light microscopic observation.
What are the applications of Application
XGLUC Cyclohexylammonium Salt is a chromogenic substrate for β-glucuronidase gene detection
General Description
X-GlcA is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. X-GlcA is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Biochem/physiol Actions
5-bromo-4-chloro-3-indolyl-β-D-glucuronide sodium salt (X-GlcA) is a colorless substrate. The pre-immobilized X-GlcA can be converted by β-glucuronidase enzyme, synthesised by?Escherichia coli.
Properties of 5-BROMO-4-CHLORO-3-INDOLYL B-D-
| Melting point: | 228-230°C |
| alpha | -87～-94°(20℃/D)(c=1,H2O-DMF)(calculated on the dehydrous basis) |
| Density | 1.4241 (rough estimate) |
| refractive index | 1.6110 (estimate) |
| storage temp. | -20°C |
| solubility | DMF: soluble |
| form | tablet |
| color | White to off-white or colorless |
| Stability: | Hygroscopic |
| EPA Substance Registry System | .beta.-D-Glucopyranosiduronic acid, 5-bromo-4-chloro-1H-indol-3-yl, compd. with cyclohexanamine (1:1) (114162-64-0) |
Safety information for 5-BROMO-4-CHLORO-3-INDOLYL B-D-
| Signal word | Warning |
| Pictogram(s) |
 Exclamation Mark Irritant GHS07 |
| GHS Hazard Statements |
H302:Acute toxicity,oral H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H332:Acute toxicity,inhalation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation |
| Precautionary Statement Codes |
P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P280:Wear protective gloves/protective clothing/eye protection/face protection. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. |
Computed Descriptors for 5-BROMO-4-CHLORO-3-INDOLYL B-D-
| InChIKey | PSBARFROSMJLJR-OXLZRBRISA-N |
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