5-BROMO-4-CHLORO-3-INDOLYL B-D-

Synonym(s):X-GlcA;X-glucuronide CHA salt

CAS NO.：114162-64-0
Empirical Formula: C20H26BrClN2O7
Molecular Weight: 521.79
MDL number: MFCD13185253
EINECS: 601-302-0
SAFETY DATA SHEET (SDS)
Update Date: 2026-06-16 21:18:09

5-BROMO-4-CHLORO-3-INDOLYL B-D- Structural

What is 5-BROMO-4-CHLORO-3-INDOLYL B-D-?

Chemical properties

White Solid

The Uses of 5-BROMO-4-CHLORO-3-INDOLYL B-D-

5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt has been used as a histochemical reagent in X-GLUC staining.

The Uses of 5-BROMO-4-CHLORO-3-INDOLYL B-D-

Suitable for use in selection of recombinant bacterial colonies with the lac⁺ phenotype.
X-GlcA has been used in histochemical staining of root sections for light microscopic observation.

What are the applications of Application

XGLUC Cyclohexylammonium Salt is a chromogenic substrate for β-glucuronidase gene detection

General Description

X-GlcA is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. X-GlcA is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Biochem/physiol Actions

5-bromo-4-chloro-3-indolyl-β-D-glucuronide sodium salt (X-GlcA) is a colorless substrate. The pre-immobilized X-GlcA can be converted by β-glucuronidase enzyme, synthesised by?Escherichia coli.

Properties of 5-BROMO-4-CHLORO-3-INDOLYL B-D-

Melting point:	228-230°C
alpha	-87～-94°(20℃/D)(c=1,H2O-DMF)(calculated on the dehydrous basis)
Density	1.4241 (rough estimate)
refractive index	1.6110 (estimate)
storage temp.	-20°C
solubility	DMF: soluble
form	tablet
color	White to off-white or colorless
Stability:	Hygroscopic
EPA Substance Registry System	.beta.-D-Glucopyranosiduronic acid, 5-bromo-4-chloro-1H-indol-3-yl, compd. with cyclohexanamine (1:1) (114162-64-0)

Safety information for 5-BROMO-4-CHLORO-3-INDOLYL B-D-

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H302:Acute toxicity,oral H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H332:Acute toxicity,inhalation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes	P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P280:Wear protective gloves/protective clothing/eye protection/face protection. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.