5-Bromo-3-indolyl-beta-D-galactopyranoside
Synonym(s):5-Bromo-3-indolyl β-D -galactopyranoside;Bluo-Gal;substrate for β-galactosidase
- CAS NO.：97753-82-7
- Empirical Formula: C14H16BrNO6
- Molecular Weight: 374.18
- MDL number: MFCD00063691
- EINECS: 1533716-785-6
- SAFETY DATA SHEET (SDS)
- Update Date: 2026-01-13 11:14:08
What is 5-Bromo-3-indolyl-beta-D-galactopyranoside?
Chemical properties
White to off-white powder
The Uses of 5-Bromo-3-indolyl-beta-D-galactopyranoside
5-Bromo-3-indolyl-beta-D-galactopyranoside is a substrate for beta-galactosidase that is converted, by the enzyme, into an insoluble indigoblue chromophore. It is also used in Lac gene detection systems, in immunoblotting and immunocytochemical assays.
The Uses of 5-Bromo-3-indolyl-beta-D-galactopyranoside
5-Bromo-3-indolyl-β-D-galactopyranoside is an α-galactosidase substrate which is converted to an insoluble indigo-blue chromophore darker than that released by X-GAL. It is ideal for Lac gene detection systems in immunoblotting, immunocytochemical, and histological applications. Also used as chromogenic substrate that is suitable for identification of lac-(+) bacterial colonies.
What are the applications of Application
5-Bromo-3-indolyl β-D-galactopyranoside is a chromogenic substrate
Properties of 5-Bromo-3-indolyl-beta-D-galactopyranoside
| Melting point: | 195 °C |
| Boiling point: | 646.6±55.0 °C(Predicted) |
| alpha | -34.5 º (c=1% in DMF/H2O 1:1) |
| Density | 1.824±0.06 g/cm3(Predicted) |
| storage temp. | 2-8°C |
| solubility | DMF: soluble |
| form | Powder |
| pka | 12.82±0.70(Predicted) |
| color | White |
| optical activity | [α]20/D 34.5±2.0°, c = 1% in DMF/H2O 1:1 |
| Water Solubility | Soluble in dimethylformamide (100 mg/ml), dimethylformamide/water (1:1 v/v) (10 mg/ml - clear, practically colorless solution). |
| BRN | 1550140 |
| CAS DataBase Reference | 97753-82-7(CAS DataBase Reference) |
Safety information for 5-Bromo-3-indolyl-beta-D-galactopyranoside
| Signal word | Warning |
| Pictogram(s) |
 Exclamation Mark Irritant GHS07 |
| GHS Hazard Statements |
H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation |
| Precautionary Statement Codes |
P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P271:Use only outdoors or in a well-ventilated area. P280:Wear protective gloves/protective clothing/eye protection/face protection. |
Computed Descriptors for 5-Bromo-3-indolyl-beta-D-galactopyranoside
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