4-Thiouracil

Description

4-Thiouracil is a site-specific, photoactivatable probe used to detect RNA structures and nucleic acid-nucleic acid contacts. It absorbs ultraviolet light >300 nm and, in the presence of oxygen, acts as an energy donor to produce singlet oxygen by triplet-triplet energy transfer. The highly reactive oxygen species then reacts readily with 4-thiouracil, leading to the production of uracil and uracil-6-sulfonate, which is fluorescent at a wavelength of ~390 nm. 4-Thiouracil is used as a T. gondii uracil phosphoribosyltransferase substrate to produce 4-thiouridine monophosphate, which can ultimately be incorporated into RNA.

Chemical properties

yellow powder

The Uses of 4-Thiouracil

4-Thiouracil is a derivative of Uracil (U801000), which is a nitrogenous base in RNA nucleic acid. 4-Thiouracil is used for tagging in cell type-specific RNA isolation from intact complex tissues.

The Uses of 4-Thiouracil

4-Thiouracil is suitable reagent employed in Schneider′s media for the embryos during RNA extraction. It may be employed as reagent for the Northwestern blotting technique.

What are the applications of Application

4-Thiouracil is a compound used in biosynthetic tagging in RNA analysis

Synthesis Reference(s)

Synthesis, p. 256, 1987 DOI: 10.1055/s-1987-27906