2-HYDROXY-5-NITROBENZYL BROMIDE

Synonym(s):α-Bromo-4-nitro-o-cresol;2-Bromomethyl-4-nitrophenol;Koshland I;Koshland’s Reagent I

CAS NO.：772-33-8
Empirical Formula: C7H6BrNO3
Molecular Weight: 232.03
MDL number: MFCD00007340
EINECS: 212-248-9
SAFETY DATA SHEET (SDS)
Update Date: 2025-09-25 17:15:13

2-HYDROXY-5-NITROBENZYL BROMIDE Structural

What is 2-HYDROXY-5-NITROBENZYL BROMIDE?

Chemical properties

Beige to yellow crystalline powder

The Uses of 2-HYDROXY-5-NITROBENZYL BROMIDE

2-Hydroxy-5-nitrobenzyl bromide is a chemical reagent that reacts with and modifies chemically the tryptophan portion of protein molecules..

The Uses of 2-HYDROXY-5-NITROBENZYL BROMIDE

Reagent for sulfhydryl modification.1

The Uses of 2-HYDROXY-5-NITROBENZYL BROMIDE

2-Hydroxy-5-nitrobenzyl bromide was used in covalent modification of tryptophan and tryptophan residues in monoclonal immunoglobulin. It was also used as reagent for sulfhydryl modification

What are the applications of Application

2-Hydroxy-5-nitrobenzyl bromide is A chemical reagent that reacts with and modifies chemically the tryptophan portion of protein molecules.

Synthesis Reference(s)

Journal of the American Chemical Society, 86, p. 1448, 1964 DOI: 10.1021/ja01061a045

General Description

2-Hydroxy-5-nitrobenzyl bromide is a protein modifying reagent.

Purification Methods

Crystallise the bromide from *benzene or *benzene/ligroin. It is slightly soluble in EtOH, soluble in *C6H6 and AcOH, and very soluble in ligroin. [Beilstein 6 H 367.]

Properties of 2-HYDROXY-5-NITROBENZYL BROMIDE

Melting point:	144-149 °C(lit.)
Boiling point:	383.0±32.0 °C(Predicted)
Density	1.795
refractive index	1.6090 (estimate)
storage temp.	2-8°C
solubility	chloroform: soluble25mg/mL, clear, yellow
pka	6.16±0.30(Predicted)
form	Crystalline Powder
color	Beige to light brown
BRN	1868798
CAS DataBase Reference	772-33-8(CAS DataBase Reference)
EPA Substance Registry System	Phenol, 2-(bromomethyl)-4-nitro- (772-33-8)

Safety information for 2-HYDROXY-5-NITROBENZYL BROMIDE

Signal word	Danger
Pictogram(s)	Corrosion Corrosives GHS05
GHS Hazard Statements	H314:Skin corrosion/irritation
Precautionary Statement Codes	P260:Do not breathe dust/fume/gas/mist/vapours/spray. P280:Wear protective gloves/protective clothing/eye protection/face protection. P363:Wash contaminated clothing before reuse. P303+P361+P353:IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.