REACTIVE BLUE 2

Chemical properties

dark blue powder

Preparation

1-Amino-4-bromo-9,10-dioxo-9,10-dihydroanthracene-2-sulfonic acid and 2,5-Diaminobenzenesulfonic acid condensation, again with 2,4,6-Trichloro-1,3,5-triazine condensation, and 4-Aminobenzenesulfonic acid or 3-Aminobenzenesulfonic acid condensation.

Enzyme inhibitor

This reactive dye (FWtrisodium-salt = 840.11 g/mol; lmax = 607 nm), also known as Reactive Blue 2 and Cibacron Blue F3G-A, is a sulfonated triazine often used to probe nucleotide binding sites. Procion Blue H-B is also a P2Y purinoceptor antagonist and is one of the most potent antagonists for ATPactivated channels (See also Cibacron Blue). When immobilized on a support matrix, this triazine can be used to purify proteins that bind nucleotides or nucleotide-containing coenzymes. Note: Given its tendency to bind in place of nucleotides, this agent is apt to have inhibitory effects beyond those listed below. Target(s): aldehyde reductase; alkaline phosphatase; ATP-activated channels; carbonyl reductase; choline Oacetyltransferase; choline-phosphate cytidylyltransferase; coenzyme F420 hydrogenase; ferredoxin:NADP+ oxidoreductase; glucose-6- phosphate dehydrogenase; glutathione S-transferase; hexokinase; NAD+ nucleosidase, or NADase, or NAD+ glycohydrolase; nucleotide diphosphatase; phosphoglycerate kinase; P2Y purinoceptor; thymidylate synthase; and UDP-glucose 4- epimerase.

Properties and Applications

bright blue. Blue powder, have good solubility in water, not sensitive to that fights. In dye bath of iron ion in color shade darker; Copper ions in colored light turned green. Used for cellulose fiber, polyamide, wool, silk dyeing and printing.