QUANTITATIVE RT-PCR READYMIX

The Uses of QUANTITATIVE RT-PCR READYMIX

Quantitative RT-PCR ReadyMix^? has been used in the quantification of messenger RNA (mRNA) levels of specific expressed genes by quantitative reverse-transcription polymerase chain reaction (RT-qPCR).

General Description

Quantitative RT-PCR ReadyMix^? combines Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) and JumpStart^? Taq DNA polymerase in a one-step RT-PCR kit designed for the measurement of gene expression. Tis convenient 2X ready mix includes M-MLV RT, JumpStart^? Taq DNA polymerase, 99% pure deoxynucleotides, buffer, glass passivator, and stabilizers. The JumpStart^? Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques. This kit has been formulated for fluorogenic hybridization probe-based detection methods.