NEUROPEPTIDE S (HUMAN)

Neuropeptide S human (4 pM to 1.7 nM; 48 hours) retains full agonist activity with an EC ₅₀ of 6.7 ± 2.4 nM, the binding of [ ^{125I ] Y10-hNPS to CHO cells stably expressing hNPSR is saturable with high affinity (K _d = 0.33 ± 0.12 nM). Neuropeptide S human (1 pM-3 μM; 48 hours) are used to compete with 0.15 nM [ 125I I] Y10-NPS, [ 125I I] Y10-NPS is displaceable by increasing concentrations of human NPS (IC ₅₀ = 0.42 ± 0.12 nM).}

Neuropeptide S human (0.1 nM-1 nM; icv) causes a significant increase in locomotor activity, the total distance traveled, percentage of time moving, number of rearing events, and center entries are also significantly increased in mice .