N-ALPHA-CBZ-ARG-ARG 7-AMIDO-4-METHYLCOUMARIN HYDROCHLORIDE

The Uses of N-ALPHA-CBZ-ARG-ARG 7-AMIDO-4-METHYLCOUMARIN HYDROCHLORIDE

Z-Arg-Arg-7-amido-4-methylcoumarin hydrochloride has been used:

• as a cathepsin B substrate in zymography analysis of protease in feces extract.
• in cathepsin B activity assay.
• as a fluorogenic peptide substrate to determine the matrix metalloproteinase enzyme activity.

General Description

Human cathepsin B (GenBank Accession No. NM_001908), CD33 signal peptide(amino acids 1-16) + Cathepsin B (amino acids 18-339), with C-terminal HIS tag, MW = 43 kDa, expressed in FreeStyle 293-F cells. Cathepsin B is a cysteine protease, found in the lysosome of normal cells and tissues. Cathepsin B active human is encoded by the gene CTSB, located on human chromosome 8p23.1.

Biochem/physiol Actions

Cathepsin B Active human primarily is a preproenzyme, glycosylated on rough endoplasmic reticulum. Processing of procathepsin B to a 31 kDa, single chain active form occurs in the lysosome. It is further processed to double chain form with a 25/26 kDa heavy chain and 5 kDa heavy chain in lysosome. Cathepsin B, functions as an exopeptidase at low pH. At higher pH in endosomes or neutral pH, cathepsin B functions as endopeptidase. Cathepsin B expression is upregulated in malignant tumors and facilitates tumor invasion. Cathepsin B might reduce the production of amyloid-β peptide associated with Alzheimerβs disease. It also plays a key role in neuroprotective functions.