KAPA2G Fast HotStart ReadyMix

The Uses of KAPA2G Fast HotStart ReadyMix

KAPA2G Fast HotStart^? ReadyMix^? has been used for:

• Routine PCR
• Fast PCR
• Reverse transcription polymerase chain reaction (RT-PCR)
• Genotyping and fluorescent PCR

The Uses of KAPA2G Fast HotStart ReadyMix

KAPA2G Fast HotStart^? ReadyMix^? has been used in:

• Routine PCR
• Fast PCR
• Genotyping
• Multiplex allele-specific colony-PCR (MASC-PCR)
• Genotyping polymerase chain reaction (PCR)

General Description

KAPA2G Fast DNA Polymerase is a second-generation (2G) enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase. In addition to speed, KAPA2G Fast provides higher yields and sensitivity than competitor enzymes across a broad range of targets.
KAPA2G Fast DNA Polymerase Kits are designed for fast PCR, in which total reaction times are 20–70% shorter than those of conventional PCR assays performed with wild-type Taq DNA polymerase. This can be achieved without sacrificing reaction performance, and does not require specialized PCR consumables or thermocyclers.
KAPA2G Fast HotStart^? DNA Polymerase is supplied in a convenient 2X ReadyMix format, containing all reaction components except primers and template.

Biochem/physiol Actions

KAPA2G Fast HotStart? DNA Polymerase generated DNA fragments may be used for routine downstream analyses or applications, including restriction enzyme digestion, cloning, and sequencing. Like wild-type Taq, KAPA2G Fast has 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′→5′ exonuclease (proofreading) activity. The fidelity of KAPA2G Fast is similar to that of wild-type Taq; it has an error rate of approximately 1 error per 1.7 x 105 nucleotides incorporated.