J-14

CAS NO.：1043854-13-2
Empirical Formula: C28H25ClN4O2S
Molecular Weight: 517.04
Update Date: 2026-01-29 15:22:00

What is J-14?

Biological Activity

J14 is a reversible sulfiredoxin inhibitor with IC50 of 8.1 μM. J14 induces oxidative stress (leading to intracellular ROS accumulation) by inhibiting sulfiredoxin, which leads to cytotoxicity and cancer cell death.

in vitro

J14 (0-100 μM; 0-96 hours; A549 cells) treatment inhibits the growth of A549 cells in a concentration- and a time-dependent manner, and its half inhibitory concentration for the growth of A549 cells was 15.7 μM.
J14 (20 μM; 48-72 hours; A549 cells) treatment causes not only the release of cytochrome c into the cytosol, but also the activation of caspase-3 and caspase-9. J14 induces oxidative damage to mitochondria, resulting in caspase-mediated apoptosis.
J14 treatment significantly increases the accumulation of sulfinic peroxiredoxins and intracellular ROS. Excess accumulation of intracellular ROS causes oxidative damage, leading to cell death. J14 significantly induces cell death in A549 cells in a time-dependent manner, resulting in approximately 40% cell death in 96 hours.
J14 induces oxidative mitochondrial damage and apoptosis.

Cell Viability Assay

Cell Line:	A549 cells
Concentration:	0-100 μM
Incubation Time: < /td>	0 hour, 24 hours, 48 hours, 72 hours, 96 hours
Result:	Inhibited the growth of A549 cells in a concentration- and a time- dependent manner.

Western Blot Analysis

< /tr>

Cell Line:	A549 cells
Concentration:	20 μM
Incubation Time:	48 hours, 72 hours
Result:	Caused not only the release of cytochrome c into the cytosol, but also the activation of caspase -3 and caspase-9.

in vivo

J14 (50 mg/kg; intraperitoneal injection; daily; for 16 days; BALB/c nude female mice) treatment significantly reduces the average tumor volume. The masses and weights of the primary tumors excised from the J14-

Animal Model:	Six-week-old BALB/c nude female mice injected with A549 cells
Dosage:	50 mg/kg
Administration:	Intraperitoneal injection; daily; for 16 days
Result:	Significantly reduced the growth of human lung tumor without acute toxicity.

Properties of J-14

Boiling point:	762.9±60.0 °C(Predicted)
Density	1.42±0.1 g/cm3(Predicted)
storage temp.	Sealed in dry,Room Temperature
solubility	DMSO: 260 mg/mL (502.86 mM)
form	Solid
pka	4.08±0.10(Predicted)
color	White to off-white

Safety information for J-14

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H302:Acute toxicity,oral H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes	P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P280:Wear protective gloves/protective clothing/eye protection/face protection. P301+P312:IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell. P302+P352:IF ON SKIN: wash with plenty of soap and water. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

Computed Descriptors for J-14

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