HEXANUCLEOTIDE MIX

Synonym(s):nucleotide mix

Molecular Weight: 0
MDL number: MFCD02098742
Update Date: 2025-01-27 09:38:02

What is HEXANUCLEOTIDE MIX?

The Uses of HEXANUCLEOTIDE MIX

Hexanucleotide Mix is a mixture of hexanucleotides of all possible sequences for random-primed DNA labeling.
Labeled DNA probes with high specific activity are used in a variety of hybridization techniques:

Screening of gene libraries
Southern and northern blots
In situ hybridizations
RT-PCR
Generation of cDNA libraries
Synthesis of first-strand cDNA
in the determination of vector titer
Second strand synthesis

General Description

Convenient oligonucleotide mixture for rapid random-primed labeling of DNA with radioactive, digoxigenin- or biotin-labeled nucleotides. In this method, the complementary DNA strand is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers.

Properties of HEXANUCLEOTIDE MIX

storage temp.	-20°C
form	solution

Safety information for HEXANUCLEOTIDE MIX

Computed Descriptors for HEXANUCLEOTIDE MIX

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