Giemsa stain

Synonym(s):Azure eosin methylene blue;Azure mixture sicc. Giemsa stain;Giemsa solution

CAS NO.：51811-82-6
Empirical Formula: C14Cl1H14N3S1
Molecular Weight: 291.8
MDL number: MFCD00081642
EINECS: 257-438-2
SAFETY DATA SHEET (SDS)
Update Date: 2025-08-21 22:16:43

What is Giemsa stain?

Chemical properties

DARK GREEN TO GREENISH-BLACK FINE CRYST. POWDER

The Uses of Giemsa stain

Giemsa Stain is used as a laboratory reagent and stain for microscopy. It is also used for the histopathologic detection of malaria and other parasites such as spirochete and protozoan. The stain is also been incorporated in differential staining between bacterial and human cells. The compound has been used in combination with May-Grunwald or Wright Stain (sc-203763) as a histological hematology stain.

The Uses of Giemsa stain

Giemsa stain has been used for the staining of cells and blood films.

What are the applications of Application

Giemsa Stain is a high quality stain for chromatin and the nuclear membrane

Definition

ChEBI: Azure A is an organic chloride salt having 3-amino-7-(dimethylamino)phenothiazin-5-ium as the counterion. It is used in making azure eosin stains for blood smear staining. It has a role as a histological dye and a fluorochrome. It contains a 3-amino-7-(dimethylamino)phenothiazin-5-ium.

General Description

Modified Giemsa stain means when the pH of the Giemsa stain is changed from the usual 6.8 to 9.0.

Properties of Giemsa stain

Melting point:	300 °C(lit.)
Density	0.99
Flash point:	15 °C
storage temp.	room temp
solubility	ethanol: 1 mg/mL
form	powder
color	Greenish-gold
PH	pH (25℃) : 6.0～8.0
Water Solubility	Insoluble in water (<0.1%), methanol (5.8 mg/L), and ethanol.
CAS DataBase Reference	51811-82-6(CAS DataBase Reference)
EPA Substance Registry System	Giemsa's stain (51811-82-6)

Safety information for Giemsa stain

Signal word	Danger
Pictogram(s)	Flame Flammables GHS02 Skull and Crossbones Acute Toxicity GHS06 Health Hazard GHS08
GHS Hazard Statements	H225:Flammable liquids H370:Specific target organ toxicity, single exposure
Precautionary Statement Codes	P210:Keep away from heat/sparks/open flames/hot surfaces. — No smoking. P260:Do not breathe dust/fume/gas/mist/vapours/spray. P280:Wear protective gloves/protective clothing/eye protection/face protection. P310:Immediately call a POISON CENTER or doctor/physician. P311:Call a POISON CENTER or doctor/physician. P301+P310:IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician.