Genopure PlasMid Midi Kit

Synonym(s):plasmid isolation

Molecular Weight: 0
Update Date: 2025-01-27 09:38:02

What is Genopure PlasMid Midi Kit?

The Uses of Genopure PlasMid Midi Kit

The Genopure Plasmid Midi Kit prepares transfection-grade plasmid DNA in medium quantities (up to 100 μg plasmid) from bacterial cultures. Isolated plasmid is suitable for most molecular biology applications:

Transfection
Southern blotting
Sequencing
PCR
Restriction analysis
Cloning

General Description

Plasmid recovery was tested with 50 μg purified plasmid. The recovery was >90%, with more than 80% in supercoiled form. The yield of plasmid DNA was determined by isolating pBS from DH5a cells. From 30 mL culture volume with a density of A600 between 3 and 6, >85 μg of plasmid DNA was obtained.

The purity (checked by the ratio of A260/A280) is 1.8 + 0.2.

No RNA is detectable.

The kit components have been tested for the absence of nucleases according to current quality control procedures.

Safety information for Genopure PlasMid Midi Kit

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