FITC-Tyramide

Description

FITC-tryamide is a reagent for use in catalyzed reporter deposition (CARD) signal amplification1 protocols in a variety of immunoassays in which horseradish peroxidase catalyzes local deposition of FITC tyramide. Typically used in multiplex immunohistochemistry (IHC)2, even allowing antibodies of the same species to be used3. Also useful in fluorescent in situ hybridization (FISH)4, including plant chromosomes, which are notorious for their repetitive regions5. Can also be used in more unique ways, to detect dimerization6, or reactive oxygen species7.

References

1) Bobrow et al. (1989), Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays; J. Immunol. Methods, 125 279 2) Zhang et al. (2017), Fully automated 5-plex fluorescent immunohistochemistry with tyramide signal amplification and same species antibodies; Lab. Invest., 97 873 3) Toth and Mezey (2007), Simultaneous Visualization of Multiple Antigens With Tyramide Signal Amplification Using Antibodies From the Same Species; J. Histochem, Cytochem., 55 545 4) Riou et al. (2017), Specificity Re-evaluation of Oligonucleotide Probes for the Detection of Marine Picoplankton by Tyramide Signal Amplification-Fluorescent In Situ Hybridization; Front. Microbiol., 8 854 5) Kirov et al. (2014), Anchoring Linkage Groups of the Rosa Genetic Map to Physical Chromosomes With tyramide-FISH and EST-SNP Markers; PLoS One., 9 e95793 6) Xu et al. (2019), DNAzyme Catalyzed Tyramide Depositing Reaction for In Situ Imaging of protein Status on the Cell Surface; Theranostics, 9 1993 7) Larios et al. (2001), Oxidative Protein Cross-Linking Reactions Involving L-tyrosine in Transforming Growth factor-beta1-stimulated Fibroblasts; J. Biol. Chem., 276 17437