FastStart Universal Probe Master (Rox)

Synonym(s):qPCR

Molecular Weight: 0
Update Date: 2025-01-27 09:38:02

What is FastStart Universal Probe Master (Rox)?

The Uses of FastStart Universal Probe Master (Rox)

FastStart^? Universal Probe Master (Rox) has been used:

in TaqMan^? quantitative real-time polymerase chain reaction (qRT-PCR) reactions for the quantification of endogenous miRNAs, such as MIR376B, MIR376A and MIR181A
in reverse transcriptase (RT-PCR) to study tumor necrosis factor (TNF) expression in whole synovial tissue of undifferentiated peripheral inflammatory arthritis (UPIA) patients
for the amplification and detection of any DNA or cDNA target, including those that are GC- or AT-rich by quantitativePCR

Combine this master mix with Transcriptor First Strand cDNA Synthesis Kit (Roche) to achieve excellent results in two-step qRT-PCR.

General Description

Hot start protocols have been shown to significantly improve the specificity, sensitivity, and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of FastStart^? Taq DNA Polymerase make the modified enzyme inactive at room temperature. Therefore, there is no elongation during the period when primers can nonspecifically bind. FastStart^? Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (i.e., a preincubation step at +95°C).

Safety information for FastStart Universal Probe Master (Rox)

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