FastStart TaqManProbe Master

Synonym(s):qPCR

Molecular Weight: 0
Update Date: 2025-01-27 09:38:02

What is FastStart TaqManProbe Master?

The Uses of FastStart TaqManProbe Master

The FastStart TaqMan^? Probe Master has been used in:

quantitative, real-time DNA-detection assays
qPCR and two-step reverse transcription (RT)-qPCR
in the hydrolysis probe detection format

General Description

Hot start protocols have been shown to significantly improve the specificity, sensitivity, and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of FastStart Taq DNA Polymerase make the modified enzyme inactive at room temperature. Therefore, there is no elongation during the period when primers can nonspecifically bind. The FastStart Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (i.e., a pre-incubation step at +95°C).

Safety information for FastStart TaqManProbe Master

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