DLD1 CELLS AKT2 -/-

The Uses of DLD1 CELLS AKT2 -/-

Media Renenewal changes two to three times per week.
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture ratio: approx. 1:3 to 1:10.
The base medium for this cell line is RPMI, Cat. No. R5886. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%, L-glutamine, Cat. No. G7513, to a final concentration of 2 mM and sodium pyruvate, Cat. No. S8636, to a final concentration of 1 mM.
Cell freezing medium-DMSO 1X, Cat. No. C6164.

General Description

DLD1 Cells SMAD4 -/- are colorectal adenocarinoma, epithelial cells, from a human adult male, with a ZFN knock out modification.
This product corresponds to ATCC Cat. No. CCL-221.