DL-Dithiothreitol

Chemical properties

White solid

The Uses of DL-Dithiothreitol

Reagent for the quantitative reduction of disulfide group. Prevents the oxidation of cholecystokinin during its extraction from brain and duodenal tissues. Cleavage of disulfide bonds in proteins.

The Uses of DL-Dithiothreitol

DTT is a cell permeable protective agent for Thiol groups.

Preparation

The preparation of DL-Dithiothreitol is as follows:To a round bottom flask was charged THPP (23mg, 0.11mmol, 1.10eq) and the basic buffer solution [tris-(hydroxymethyl)-aminomethane-CaCl2 based buffer; pH 8.00; 1.0mL]. The reaction mixture was stirred at room temperature for about 5 min and then to this homogenous solution was charged trans-4,5-Dihydroxy-1,2-dithiane (15.2mg, 0.10 mmol, 1.00eq.). The reaction mixture was stirred at room temperature until the reaction was complete (about 30 min). The product was readily identified by 1H-NMR analysis.

Definition

ChEBI: D-1,4-dithiothreitol is a 1,4-dithiothreitol. It is an enantiomer of a L-1,4-dithiothreitol.

What are the applications of Application

DTT is a cell permeable protective agent for Thiol groups which keeps monothiols in the reduced state and reduces disulfides

Purification Methods

Crystallise DTT from ether and sublime it at 37o/0.005mm. It should be stored at 0o. [Beilstein 1 III 2360.]