Disodium piperazine-1,4-diethanesulphonate

Synonym(s):1,4-Piperazinediethanesulfonic acid disodium salt;Piperazine-1,4-bis(2-ethanesulfonic acid) disodium salt;PIPES disodium salt

CAS NO.：76836-02-7
Empirical Formula: C8H16N2Na2O6S2
Molecular Weight: 346.33
MDL number: MFCD00064349
EINECS: 278-562-3
SAFETY DATA SHEET (SDS)
Update Date: 2026-07-16 07:06:08

Disodium piperazine-1,4-diethanesulphonate Structural

What is Disodium piperazine-1,4-diethanesulphonate?

Chemical properties

White powder

The Uses of Disodium piperazine-1,4-diethanesulphonate

Biological buffer.

The Uses of Disodium piperazine-1,4-diethanesulphonate

PIPES Disodium is the sodium salt form of PIPES and is a biological buffer often referred to as a “Good′s” buffer. The pKa of PIPES is 6.8 which makes it ideal for biological applications at physiological conditions. PIPES Disodium is considered to be non-toxic to culture cell lines, highly water soluble and provides high-solution clarity.

PIPES Disodium can be used in a wide array of biological applications including as a component in media formulations. Specific applications include buffers for cell culture and diagnostic reagent production.

What are the applications of Application

PIPES disodium salt is piperazine-N,N′-bis(2-ethanesulfonic acid), a buffering agent used in biological applications

Definition

ChEBI: An organic salt, being the disodium salt of 2,2'-piperazine-1,4-diylbisethanesulfonic acid (PIPES), a buffering agent.

Flammability and Explosibility

Not classified

Purification Methods

It crystallises from water and EtOH. [Beilstein 23/2 V 380.]

Properties of Disodium piperazine-1,4-diethanesulphonate

Density	1.72[at 20℃]
vapor pressure	0Pa at 20℃
storage temp.	Inert atmosphere,Room Temperature
solubility	H₂O: 0.5 g/mL, clear, colorless
form	Powder or Crystalline Powder
color	White
pka	6.8(at 25℃)
PH Range	6.1 - 7.5
Water Solubility	855g/L at 19.8℃
Merck	14,7479
BRN	9453872
CAS DataBase Reference	76836-02-7(CAS DataBase Reference)
EPA Substance Registry System	1,4-Piperazinediethanesulfonic acid, disodium salt (76836-02-7)

Safety information for Disodium piperazine-1,4-diethanesulphonate

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H302:Acute toxicity,oral H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes	P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.