DIHYDROETHIDIUM

CAS NO.：104821-25-2
Empirical Formula: C21H21N3
Molecular Weight: 315.41
MDL number: MFCD00192079
SAFETY DATA SHEET (SDS)
Update Date: 2026-01-13 11:18:02

What is DIHYDROETHIDIUM?

Chemical properties

DIHYDROETHIDIUM is pink to purple fine crystalline powder

The Uses of DIHYDROETHIDIUM

DIHYDROETHIDIUM is redox indicator. Blue fluorescence until oxidized to ethidium. Can be used for detecting superoxide radical in cells, tissues and organisms.

The Uses of DIHYDROETHIDIUM

Dihydroethidium, also known as hydroethidine, is a dye that can permeate viable cells and accumulate in the nucleus after it is dehydrogenated to ethidium bromide and is used to measure oxygen reduction in phagocytes during respiratory burst. In the presence of an oxidant it will fluoresce and has been used as a dye in flow cytometry. Studies have used Dihydroethidium to study alveolar macrophages, blood leukocytes, and epithelial cells, among others, and has be used to measure intracellular H2O2 and O2-, and reactive oxygen. Dyes and metabolites.

What are the applications of Application

Dihydroethidium is an effective dye used to measure oxygen reduction in phagocytes during respiratory burst

Properties of DIHYDROETHIDIUM

Boiling point:	580.4±50.0 °C(Predicted)
Density	1?+-.0.06 g/cm3(Predicted)
storage temp.	−20°C
solubility	acetonitrile: soluble
form	powder
pka	5.30±0.40(Predicted)
color	Pink to dark brown
λmax	355 nm
BRN	5107482
Biological Applications	Apoptosis assay; generating and detecting reactive oxygen species (ROS); detecting nucleic acids,cells; measuring respiratory burst; superoxide indicator; viability assay