Dihydroethidium
- CAS NO.：104821-25-2
- Empirical Formula: C21H21N3
- Molecular Weight: 315.41
- MDL number: MFCD00192079
- SAFETY DATA SHEET (SDS)
- Update Date: 2026-09-12 18:52:05
What is Dihydroethidium?
Chemical properties
DIHYDROETHIDIUM is pink to purple fine crystalline powder
The Uses of Dihydroethidium
DIHYDROETHIDIUM is redox indicator. Blue fluorescence until oxidized to ethidium. Can be used for detecting superoxide radical in cells, tissues and organisms.
The Uses of Dihydroethidium
Dihydroethidium, also known as hydroethidine, is a dye that can permeate viable cells and accumulate in the nucleus after it is dehydrogenated to ethidium bromide and is used to measure oxygen reduction in phagocytes during respiratory burst. In the presence of an oxidant it will fluoresce and has been used as a dye in flow cytometry. Studies have used Dihydroethidium to study alveolar macrophages, blood leukocytes, and epithelial cells, among others, and has be used to measure intracellular H2O2 and O2-, and reactive oxygen. Dyes and metabolites.
What are the applications of Application
Dihydroethidium is an effective dye used to measure oxygen reduction in phagocytes during respiratory burst
Properties of Dihydroethidium
| Boiling point: | 580.4±50.0 °C(Predicted) |
| Density | 1?+-.0.06 g/cm3(Predicted) |
| storage temp. | −20°C |
| solubility | acetonitrile: soluble |
| form | powder |
| pka | 5.30±0.40(Predicted) |
| color | Pink to dark brown |
| λmax | 355 nm |
| BRN | 5107482 |
| Biological Applications | Apoptosis assay; generating and detecting reactive oxygen species (ROS); detecting nucleic acids,cells; measuring respiratory burst; superoxide indicator; viability assay |
Safety information for Dihydroethidium
Computed Descriptors for Dihydroethidium
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