DIG-HIGH PRIME

Synonym(s):DIG system

Molecular Weight: 0
MDL number: MFCD02098174
Update Date: 2025-01-27 09:38:02

What is DIG-HIGH PRIME?

The Uses of DIG-HIGH PRIME

DIG-High Prime-labeled DNA probes has been used in a variety of hybridization techniques :

in Southern blots
in northern blots
in dot/slot blots
for screening of gene libraries
in In situ hybridizations

Due to highly specific and sensitive detection systems, DIG-labeled DNA probes can be used for single-copy gene detection in 1μg total human DNA. The use of the alkali-labile form of DIG-dUTP, in which the digoxigenin moiety is connected to the spacer arm via an alkali-labile ester bond, enables easier and more efficient stripping and reprobing of blots.

General Description

DIG-High Prime has enzyme and nucleotide mixture for rapid random-primed labeling of DNA with Digoxigenin-11-dUTP. In this method, the complementary DNA strand of denatured DNA is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers. DIG-labeled probes are generated at high yield within one hour or after overnight incubation.

Properties of DIG-HIGH PRIME

storage temp.	-20°C
form	solution

Safety information for DIG-HIGH PRIME

Computed Descriptors for DIG-HIGH PRIME

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