DAF-FM
Synonym(s):3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein;4-Amino-5-methylamino- 2′,7′-difluorofluorescein;4-Amino-5-methylamino-2′,7′-difluorescein;DAF-FM - CAS 254109-20-1 - Calbiochem;Diaminofluorescein-FM
- CAS NO.：254109-20-1
- Empirical Formula: C21H14F2N2O5
- Molecular Weight: 412.34
- MDL number: MFCD03453541
- SAFETY DATA SHEET (SDS)
- Update Date: 2026-05-28 02:07:55
What is DAF-FM?
The Uses of DAF-FM
DAF-FM is important reagent for quantitating low concentrations of nitric oxide in solution. This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotrizole.
The Uses of DAF-FM
DAF-FM has been used in the visualization of nitric oxide (NO) in root sections of wheat seedlings. It has also been used to label neural crest (NC) cells.
The Uses of DAF-FM
DAF-FM solution has been used as a fluorescent probe for detecting nitric oxide (NO) from plant samples.
What are the applications of Application
DAF-FM is a photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM
Biochem/physiol Actions
DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, DAF-FM, but it does not need to be activated by cytosolic enzymes.
Properties of DAF-FM
| Melting point: | 265 ℃ |
| storage temp. | -20°C |
| solubility | DMSO: >1mg/mL |
| pka | 8.19 ± 0.20, most acidic, temperature:
25 °C; 4.23 ± 0.20, most basic(at 25℃) |
| form | Solid |
| color | clear colorless to yellow |
| λmax | 487 nm, 495 nm |
| Biological Applications | Nitric oxide indicator; detecting microorganisms; measuring Ga i-coupled or Gao-coupled receptors activity |
Safety information for DAF-FM
Computed Descriptors for DAF-FM
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