Coumarin 151

Synonym(s):Coumarin 151

CAS NO.：53518-15-3
Empirical Formula: C10H6F3NO2
Molecular Weight: 229.16
MDL number: MFCD00006858
EINECS: 258-599-1
SAFETY DATA SHEET (SDS)
Update Date: 2026-01-13 11:19:34

What is Coumarin 151?

Chemical properties

Coumarin 151 is yellow powder

The Uses of Coumarin 151

Coumarin 151 is used to prepare peptidase substrates and for the synthesis of substrates for the fluorometric assay of proteolytic enzymes in biological fluids.

The Uses of Coumarin 151

7-Amino-4-(trifluoromethyl)coumarin is used in the synthesis of substrates for the fluorometric assay of proteolytic enzymes in biological fluids. It is suitable as laser dye.

Definition

ChEBI: 7-amino-4-(trifluoromethyl)coumarin is a member of 7-aminocoumarins. It has a role as a fluorochrome.

What are the applications of Application

7-Amino-4-trifluoromethylcoumarin is for the fluorometric assay of proteolytic enzymes in biological fluids

Purification Methods

Purify the coumarin by column chromatography on a C18 column, elute with acetonitrile/0.01M H2O/HCl (1:1), and recrystallise it from isopropanol. Alternatively, it is eluted from a silica gel column with CH2Cl2, or by extracting a CH2Cl2 solution (4g/L) with 1M aqueous NaOH (3 x 0.1L), followed by drying (MgSO4), filtration and evaporation. [Bissell J Org Chem 45 2283 1980, Zimmermann et al. Anal Biochem 70 258 1976.]

Properties of Coumarin 151

Melting point:	221-222 °C(lit.)
Boiling point:	314.6±42.0 °C(Predicted)
Density	1.4096 (estimate)
storage temp.	under inert gas (nitrogen or Argon) at 2–8 °C
form	Powder
pka	1.12±0.20(Predicted)
color	Yellow
Water Solubility	Soluble in dimethyl sulfoxide (25 mg/ml), dimethyl formamide (25 mg/ml), and methanol (25 mg/ml). Insoluble in water.
BRN	4456797
CAS DataBase Reference	53518-15-3(CAS DataBase Reference)
EPA Substance Registry System	2H-1-Benzopyran-2-one, 7-amino-4-(trifluoromethyl)- (53518-15-3)

Safety information for Coumarin 151

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes	P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P264:Wash hands thoroughly after handling. P264:Wash skin thouroughly after handling. P271:Use only outdoors or in a well-ventilated area. P280:Wear protective gloves/protective clothing/eye protection/face protection. P302+P352:IF ON SKIN: wash with plenty of soap and water. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.