COELENTERAZINE H

Synonym(s):2-Deoxycoelenterazine;CLZN-h

CAS NO.：50909-86-9
Empirical Formula: C26H21N3O2
Molecular Weight: 407.46
MDL number: MFCD00798394
SAFETY DATA SHEET (SDS)
Update Date: 2026-03-17 20:01:48

What is COELENTERAZINE H?

Description

Coelenterazine-h (50909-86-9) may be used as a substrate in gene reporter assays, live cell assays, and biochemical assays (ELISA, BRET etc). Renilla luciferase catalyzes coelenterazine oxidation by oxygen to produce light. Coelenterazine-h produces 10-fold higher light output than native coelenterazine. Coelenterazine-h also undergoes autoluminescence, which is enhanced by superoxide anion and peroxynitrite in cells.

The Uses of COELENTERAZINE H

Coelenterazine h, is a derivative of Coelenterazine (C636000), and iis more sensitive to Ca2+ than is the native complex, thus providing a valuable tool for measuring small changes in Ca2+ concentrations.

What are the applications of Application

Coelenterazine-h is an intracellular luminophore for measuring superoxide and Ca2+ in cells transfected with apoaequorin cDNA

Definition

ChEBI: Renilla luciferin is a member of phenols and an imidazopyrazine. It has a role as a luciferin. It derives from a hydride of an imidazo[1,2-a]pyrazine.

General Description

Coelenterazine is an imidazopyrazinone derivative. It is a substrate for bioluminescence in many luminous marine organisms.

Biochem/physiol Actions

10 times higher luminescence intensity than native coelenterazine.

References

1) Shimomura?et al. (1989),?Semi-synthetic aequorins with improved sensitivity to Ca2+ ions; Biochem. J.,?261?913 2) Nakajima-Shimada?et al.?(1991),?Monitoring of intracellular calcium in Saccharomyces cerevisiae with an apoaequorin cDNA expression system; Proc. Natl. Acad. Sci. USA,?88?6878 3) Inouye?et al. (1992),?Monitoring gene expression in Chinese hamster ovary cells using secreted apoaequorin; Anal. Biochem.,?201?114

Properties of COELENTERAZINE H

Melting point:	>200℃
Boiling point:	593.5±60.0 °C(Predicted)
Density	1.26±0.1 g/cm3(Predicted)
storage temp.	-20°C
solubility	Soluble in methanol, ethanol
form	solid
pka	10.50±0.30(Predicted)
color	Yellow
λmax	437nm
Stability:	Stable for 1 year from ate of purchase as supplied. Solutions in DMSO or methanol may be stored at -20°C for up to 1 month.
Biological Applications	Calcium indicator; assaying luminescent enzyme; measuring luciferase activity; as a substrate for luciferase; screening HIV-1 protease inhibitors; bioluminescence resonance energy transfer (BRET) detection system

Safety information for COELENTERAZINE H

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes	P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P264:Wash hands thoroughly after handling. P264:Wash skin thouroughly after handling. P271:Use only outdoors or in a well-ventilated area. P280:Wear protective gloves/protective clothing/eye protection/face protection. P302+P352:IF ON SKIN: wash with plenty of soap and water. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.