CITRATE SYNTHASE
Synonym(s):Citrate condensing enzyme;Citrate oxaloacetate lyase (CoA-acetylating)
- CAS NO.：9027-96-7
- Empirical Formula: C6H5O7-3
- Molecular Weight: 189.0997
- MDL number: MFCD00130808
- EINECS: 232-821-7
- Update Date: 2025-10-23 17:25:30
What is CITRATE SYNTHASE?
The Uses of CITRATE SYNTHASE
Citrate Synthase from porcine heart has been used:
- to inject newt egg for determining its importance in egg activation at fertilization
- in the preparation of reaction mix to determine pyruvate carboxylase enzyme activity
- to examine whether it can induce a [Ca2+]?increase and egg activation in unfertilized eggs
Definition
An enzyme that catalyzes the condensation of oxaloacetic acid and acetyl-CoA to yield citric acid and coenzyme A.
Biochem/physiol Actions
Citrate synthase catalyses the conversion of citrate to acetyl-CoA in the presence of coenzyme-A with the release of H2O and oxaloacetate. The enzyme has a molecular weight of 85 kDa and a pI of 6.1-6.6. It is inhibited by fluoroacetyl-CoA, palmitoyl-CoA, and citroyl-CoA. It is also inhibited when it is acetylated by acetic anhydride or iodinated by iodine.
Properties of CITRATE SYNTHASE
| storage temp. | 2-8°C |
| solubility | H2O: soluble1.0mg/mL, clear |
| form | ammonium sulfate suspension |
Safety information for CITRATE SYNTHASE
Computed Descriptors for CITRATE SYNTHASE
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