Basic Green 4
Synonym(s):N,N,N′,N′-Tetramethyl-4,4′-diaminotriphenylcarbenium chloride;N,N,N′,N′-Tetramethyl-4,4′-diaminotriphenylcarbenium oxalate;Basic Green 4;Malachite Green;
- CAS NO.：2437-29-8
- Empirical Formula: C52H54N4O12
- Molecular Weight: 927
- MDL number: MFCD00011766
- EINECS: 219-441-7
- SAFETY DATA SHEET (SDS)
- Update Date: 2026-01-13 11:19:52
What is Basic Green 4?
Chemical properties
dark green crystals with a metallic luster
The Uses of Basic Green 4
Biological stain, dye reagent, acid base indicator: pH 0.0 yellow, 2.0 green;11.6 green, 14 colorlessMalachite Green oxalate is used as the staining solution for embedded botanical material and as a spore stain. It is also used in studying the photocatalytic degradation with TiO2/WO3. It is employed for determining phosphate in serum and as a reference material in analytical chemistry for the quantification. It is also used as a pH indicator between pH 0.2 - 1.8. It is used as a dye for materials such as silk, leather, and paper.
The Uses of Basic Green 4
Malachite Green oxalate salt has been used:
- in the preparation of Alexander staining solution for cell viability test
- in malachite green-ammonium molybdate assay for determining the adenosine triphosphatase (ATPase) activity of binding immunoglobulin protein (BiP)
- as a hydrophobic ion, to study the label-free molecular transport in the living cell membranes of Escherichia coli in real time by second-harmonic generation (SHG) method
Definition
A salt or ester of oxalicacid.
General Description
Malachite Green is used for bacterial spore staining by Schaeffer and Fulton`s method. It can also be used in place of methyl green in the Pappenheim stain, when combined with the Gram stain.
Biochem/physiol Actions
Malachite green is used in the treatment of fungal and parasitic infections in fish.
Properties of Basic Green 4
| Melting point: | 164°C (dec.) |
| storage temp. | room temp |
| solubility | 110g/l |
| form | Powder/Solid |
| Colour Index | 42000 |
| pka | 6.9(at 25℃) |
| color | S. No.: 754 |
| PH | 2.4 (10g/l, H2O, 24℃) |
| PH Range | 0.0(YELLOW)-2.0(GREEN); 11.0(GREEN)-13.5(CORLORLESS) |
| Water Solubility | 60 g/L (20 ºC) |
| λmax | 614 nm, 425 nm |
| Merck | 14,5699 |
| BRN | 3644550 |
| Stability: | Stable. Substances to be avoided include strong oxidizing agents. |
| Biological Applications | Detecting bacterial growth; treating fish diseases,malignant neoplasm |
| CAS DataBase Reference | 2437-29-8(CAS DataBase Reference) |
| EPA Substance Registry System | Malachite green oxalate (2437-29-8) |
Safety information for Basic Green 4
| Signal word | Danger |
| Pictogram(s) |
 Corrosion Corrosives GHS05  Skull and Crossbones Acute Toxicity GHS06  Health Hazard GHS08  Environment GHS09 |
| GHS Hazard Statements |
H301:Acute toxicity,oral H318:Serious eye damage/eye irritation H410:Hazardous to the aquatic environment, long-term hazard |
| Precautionary Statement Codes |
P201:Obtain special instructions before use. P202:Do not handle until all safety precautions have been read and understood. P273:Avoid release to the environment. P280:Wear protective gloves/protective clothing/eye protection/face protection. P301+P310:IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. |
Computed Descriptors for Basic Green 4
| InChIKey | CNYGFPPAGUCRIC-UHFFFAOYSA-L |
Basic Green 4 manufacturer
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