Basic Green 4

Synonym(s):N,N,N′,N′-Tetramethyl-4,4′-diaminotriphenylcarbenium chloride;N,N,N′,N′-Tetramethyl-4,4′-diaminotriphenylcarbenium oxalate;Basic Green 4;Malachite Green;

CAS NO.：2437-29-8
Empirical Formula: C52H54N4O12
Molecular Weight: 927
MDL number: MFCD00011766
EINECS: 219-441-7
SAFETY DATA SHEET (SDS)
Update Date: 2025-12-04 14:24:59

What is Basic Green 4?

Chemical properties

dark green crystals with a metallic luster

The Uses of Basic Green 4

Biological stain, dye reagent, acid base indicator: pH 0.0 yellow, 2.0 green;11.6 green, 14 colorlessMalachite Green oxalate is used as the staining solution for embedded botanical material and as a spore stain. It is also used in studying the photocatalytic degradation with TiO2/WO3. It is employed for determining phosphate in serum and as a reference material in analytical chemistry for the quantification. It is also used as a pH indicator between pH 0.2 - 1.8. It is used as a dye for materials such as silk, leather, and paper.

The Uses of Basic Green 4

Malachite Green oxalate salt has been used:

in the preparation of Alexander staining solution for cell viability test
in malachite green-ammonium molybdate assay for determining the adenosine triphosphatase (ATPase) activity of binding immunoglobulin protein (BiP)
as a hydrophobic ion, to study the label-free molecular transport in the living cell membranes of Escherichia coli in real time by second-harmonic generation (SHG) method

Definition

A salt or ester of oxalicacid.

General Description

Malachite Green is used for bacterial spore staining by Schaeffer and Fulton`s method. It can also be used in place of methyl green in the Pappenheim stain, when combined with the Gram stain.

Biochem/physiol Actions

Malachite green is used in the treatment of fungal and parasitic infections in fish.

Properties of Basic Green 4

Melting point:	164°C (dec.)
storage temp.	room temp
solubility	110g/l
form	Powder/Solid
Colour Index	42000
pka	6.9(at 25℃)
color	S. No.: 754
PH	2.4 (10g/l, H2O, 24℃)
PH Range	0.0(YELLOW)-2.0(GREEN); 11.0(GREEN)-13.5(CORLORLESS)
Water Solubility	60 g/L (20 ºC)
λmax	614 nm, 425 nm
Merck	14,5699
BRN	3644550
Stability:	Stable. Substances to be avoided include strong oxidizing agents.
Biological Applications	Detecting bacterial growth; treating fish diseases,malignant neoplasm
CAS DataBase Reference	2437-29-8(CAS DataBase Reference)
EPA Substance Registry System	Malachite green oxalate (2437-29-8)

Safety information for Basic Green 4

Signal word	Danger
Pictogram(s)	Corrosion Corrosives GHS05 Skull and Crossbones Acute Toxicity GHS06 Health Hazard GHS08 Environment GHS09
GHS Hazard Statements	H301:Acute toxicity,oral H318:Serious eye damage/eye irritation H410:Hazardous to the aquatic environment, long-term hazard
Precautionary Statement Codes	P201:Obtain special instructions before use. P202:Do not handle until all safety precautions have been read and understood. P273:Avoid release to the environment. P280:Wear protective gloves/protective clothing/eye protection/face protection. P301+P310:IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.