ALPHA-KETOGLUTARATE DEHYDROGENASE

Synonym(s):Multienzyme 2-oxoglutarate dehydrogenase complex

CAS NO.：9031-02-1
Molecular Weight: 0
MDL number: MFCD00130490
Update Date: 2026-01-13 11:13:15

What is ALPHA-KETOGLUTARATE DEHYDROGENASE?

The Uses of ALPHA-KETOGLUTARATE DEHYDROGENASE

α-Ketoglutarate Dehydrogenase from the porcine heart has been used:

to study the reversal of nitration by glutathione (GSH) in peroxynitrite-treated cells
to measure its activity by Spectramax M5 microplate spectrofluorimeter using heart mitochondria
as a positive control to evaluate its activity in by Spectramax GEMINI EM fluorescence microplate reader using mice neurons

General Description

α-Ketoglutarate dehydrogenase (α-KGDH) is a multienzyme complex localized to the mitochondria. This integrated enzyme is made up of many units of thiamine pyrophosphate-dependent dehydrogenase (E1), dihydrolipoamide dehydrogenase (E3), and dihydrolipoamide succinyl transferase (E2).

Biochem/physiol Actions

α-Ketoglutarate dehydrogenase (α-KGDH) is a key enzyme of bioenergetic processes and a controlling unit of metabolic flux through the Krebs cycle or tricarboxylic acid (TCA) cycle. It catalyzes the oxidative decarboxylation of α-ketoglutarate (KG) to succinyl-CoA by releasing reduced nicotinamide adenine dinucleotide (NADH). It is the rate-limiting reaction of the TCA cycle. This reaction contributes to the electrons of the respiratory chain and requires thiamine pyrophosphate as a cofactor. The reduction of NAD (nicotinamide adenine dinucleotide) is observed to determine its reaction rate. α-KGDH from porcine has an optimum pH range of 6.6–7.4. This enzyme is inhibited by oxidative stress and results in a metabolic deficiency. However, α-KGDH is also known to produce reactive oxygen species (ROS) leading to oxidative stress. Defective or limited levels of α-KGDH cause several neurodegenerative diseases such as Alzheimer′s disease.

Properties of ALPHA-KETOGLUTARATE DEHYDROGENASE

storage temp.	-20°C
form	buffered aqueous glycerol solution

Safety information for ALPHA-KETOGLUTARATE DEHYDROGENASE

GHS Hazard Statements	H412:Hazardous to the aquatic environment, long-term hazard
Precautionary Statement Codes	P273:Avoid release to the environment. P501:Dispose of contents/container to..…

Computed Descriptors for ALPHA-KETOGLUTARATE DEHYDROGENASE

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