7-nitro-1-methyl-1H-benzo[d][1,3]oxazine-2,4-dione

Synonym(s):1M7;1-methyl-7-nitro-2H-3,1-Benzoxazine-2,4(1H)-dione;Reagent for RNA SHAPE-MaP;RNA SHAPE probe

CAS NO.：73043-80-8
Empirical Formula: C9H6N2O5
Molecular Weight: 222.15
MDL number: MFCD18432729
SAFETY DATA SHEET (SDS)
Update Date: 2026-07-16 07:06:08

7-nitro-1-methyl-1H-benzo[d][1,3]oxazine-2,4-dione Structural

What is 7-nitro-1-methyl-1H-benzo[d][1,3]oxazine-2,4-dione?

The Uses of 7-nitro-1-methyl-1H-benzo[d][1,3]oxazine-2,4-dione

1-Methyl-7-nitroisatoic anhydride (1M7) is used as an in vivo SHAPE-MaP reagent for live cell RNA structure analysis at single nucleotide resolution. SHAPE -- or selective 2′-hydroxyl acylation analyzed by primer extension -- uses small, electrophilic chemical probes such as 1M7 to react with the 2′-hydroxyl group and provides insight to RNA structure. When combined with mutational profiling (MaP), quantitative nucleotide measurements are possible for entire transciptomes. Together, these methods deepen the understanding of RNA interactions and regions that may be exploited for design of RNA-targeting small-molecule drugs.

Definition

ChEBI: A 3,1-benzoxazin-1,4-dione having an N-methyl substituent and a nitro group at the 7-position.

Storage

Store at -20°C

Properties of 7-nitro-1-methyl-1H-benzo[d][1,3]oxazine-2,4-dione

Melting point:	203-206 °C
Boiling point:	395.4±44.0 °C(Predicted)
Density	1.551±0.06 g/cm3(Predicted)
storage temp.	2-8°C
solubility	Chloroform (Slightly, Heated)
form	Solid
pka	-4.82±0.20(Predicted)
color	Yellow to Dark Yellow
Stability:	Moisture Sensitive

Safety information for 7-nitro-1-methyl-1H-benzo[d][1,3]oxazine-2,4-dione

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H302:Acute toxicity,oral
Precautionary Statement Codes	P280:Wear protective gloves/protective clothing/eye protection/face protection. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.