5(6)-Carboxyfluorescein
Synonym(s):5(6)-Carboxyfluorescein;5(6)-FAM;5,6-CF
- CAS NO.：72088-94-9
- Empirical Formula: C42H24O14
- Molecular Weight: 752.63
- MDL number: MFCD00151081
- EINECS: 276-331-1
- SAFETY DATA SHEET (SDS)
- Update Date: 2026-03-13 23:13:44
What is 5(6)-Carboxyfluorescein?
Description
5(6)-Carboxyfluorescein is a mixture of 5-carboxy and 6-carboxy derivatives of fluorescein. It is commonly used to label biomolecules through a reaction involving the carboxyl group. Unlike its succinimidyl ester derivative CFSE , 5(6)-carboxyfluorescein is membrane impermeant. As a result, it can be used in studies of membrane permeability. 5(6)-Carboxyfluorescein displays excellent fluorescence (excitation/emission at 492/514 nm, respectively), and its excitation maximum closely matches the 488 nm spectral line of argon-ion lasers.
Chemical properties
Dark-Orange to Brown Solid
The Uses of 5(6)-Carboxyfluorescein
As a popular probe of determination of intracellular pH, 5(6)-Carboxyfluoresceincan be used as useful reagent for the preparation of hydrolytically stable fluorescent conjugates and a useful starting material for the synthesis of other fluorescein-derives reagent
The Uses of 5(6)-Carboxyfluorescein
A popular probe of determination of intracellular pH.
The Uses of 5(6)-Carboxyfluorescein
5(6)-FAM, A useful reagent for the preparation of hydrolytically stable fluorescent conjugates and is a useful starting material for the synthesis of other fluorescein-derives reagent.
What are the applications of Application
5(6)-Carboxyfluorescein is used in the preparation of fluorescent peptide and protein conjugates
What are the applications of Application
5(6)-carboxyfluorescein, mixed isomers is a pH indicator
Definition
ChEBI: 5(6)-carboxyfluorescein is a commercially available mixture of 5-carboxyfluorescein and 6-carboxyfluorescein isomers. A fluorescent dye, it is commonly used as a tracer agent. It has a role as a tracer and a fluorescent dye. It contains a 5-carboxyfluorescein and a 6-carboxyfluorescein.
Solubility in organics
5(6)-Carboxyfluorescein is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide. The solubility of 5(6)-carboxyfluorescein in these solvents is approximately 5, 0.5, and 1 mg/ml, respectively.
Properties of 5(6)-Carboxyfluorescein
| Melting point: | 275 °C |
| Boiling point: | 464.9°C (rough estimate) |
| Density | 1.3418 (rough estimate) |
| refractive index | 1.6380 (estimate) |
| storage temp. | Keep in dark place,Inert atmosphere,Room temperature |
| solubility | DMSO (Sparingly), Methanol (Slightly, Heated) |
| form | Solid |
| color | Light Yellow to Dark Brown |
| Water Solubility | slightly soluble |
| EPA Substance Registry System | Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylic acid, 3',6'-dihydroxy-3-oxo- (72088-94-9) |
Safety information for 5(6)-Carboxyfluorescein
| Signal word | Warning |
| Pictogram(s) |
 Exclamation Mark Irritant GHS07 |
| GHS Hazard Statements |
H319:Serious eye damage/eye irritation |
| Precautionary Statement Codes |
P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. |
Computed Descriptors for 5(6)-Carboxyfluorescein
| InChIKey | CPRVUBZYHZGASQ-UHFFFAOYSA-N |
| SMILES | C12(OC(=O)C3C=CC(C(=O)O)=CC1=3)C1=CC=C(O)C=C1OC1C=C(O)C=CC2=1.C12(OC(=O)C3C=C(C(=O)O)C=CC1=3)C1=CC=C(O)C=C1OC1C=C(O)C=CC2=1 |
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