EC 1.14.14.3
Synonym(s):Bacterial Luciferase;Luciferase from Photobacterium fischeri
- CAS NO.：9014-00-0
- Empirical Formula: NA
- Molecular Weight: 0
- MDL number: MFCD00131545
- EINECS: 232-751-7
- Update Date: 2026-03-13 15:00:32
What is EC 1.14.14.3?
The Uses of EC 1.14.14.3
Luciferase may be used:
- to treat mammary tumor cryosections for bioluminescence imaging studies
- as a component of luciferase reaction mixture
- in bioluminescence assay membrane preparations of Zymomonas mobilis
The Uses of EC 1.14.14.3
Luciferase from Vibrio fischeri has been used in a study to assess kinetics of light emission and oxygen consumption by bioluminescent bacteria. It has also been used in a study to investigate the sensitivity of dark mutants of various strains of luminescent bacteria to reactive oxygen species.
The Uses of EC 1.14.14.3
Luciferase has been used as a component in Mix B respiration medium for the determination of the ATP synthetic activity of the H+-ATP synthase. It has also been used in thermal aggregation to assay CnoX (YbbN) activity.
General Description
Alkanal reduced FMN-oxygen oxidoreductase (1-hydroxylating, luminescing)
Biochem/physiol Actions
Luciferase catalyzes the conversion of reduced flavin mononucleotide in the presence of oxygen and long-chain aldehyde to flavin mononucleotide, visible light and a fatty acid. It is a reporter enzyme for sensitive quantitation. The lucifearse fusion proteins have been useful in many protein based interaction studies especially in living cells.
Properties of EC 1.14.14.3
| storage temp. | -20°C |
| form | powder |
| color | White to light yellow |
| Water Solubility | Water: 50 mg/mL |
| EPA Substance Registry System | Luciferase (9014-00-0) |
Safety information for EC 1.14.14.3
Computed Descriptors for EC 1.14.14.3
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