4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE
Synonym(s):4-Nitrophenyl phosphate disodium salt hexahydrate;di-Sodium 4-nitrophenyl phosphate;Disodium 4-nitrophenyl phosphate hexahydrate;Phosphatase substrate;pNPP disodium hexahydrate
- CAS NO.：333338-18-4
- Empirical Formula: C6H9NNaO7P
- Molecular Weight: 261.1
- MDL number: MFCD00066288
- EINECS: 224-246-5
- SAFETY DATA SHEET (SDS)
- Update Date: 2026-06-17 18:53:45
What is 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE?
Chemical properties
White to off-white powder
The Uses of 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE
4-Nitrophenyl phosphate disodium salt hexahydrate has been used in:
- enzyme-linked immunosorbent assay (ELISA) to visualize glycoprotein VI (GPVI) binding to collagen type I (Col I)
- enzymatic color reaction for semi-quantitative measurement of allergen-specific IgE by ELISA
- alkaline phosphatase assay
The Uses of 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE
Phosphatase substrate has been used in the chromogenic detection of adsorbed rabbit IgG to determine coating efficiency.
The Uses of 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE
Substrate for alkaline and acid phosphatase determinations4-Nitrophenyl phosphate disodium salt hexahydrate is a chromogenic substrate used in the determination of acid and alkaline phosphatases.
General Description
Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.
Biochem/physiol Actions
4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP yields p-nitrophenol. pNPP is a common chromogenic substrate for alkaline phosphatase.
Properties of 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE
| Melting point: | >300 °C(lit.) |
| storage temp. | -20°C |
| solubility | H2O: 25 mg/mL at 20 °C, clear, yellow-green |
| form | tablets |
| color | white to pale yellow |
| PH | pH (50g/l, 25℃) : 8.0～9.0 |
| Water Solubility | almost transparency |
| BRN | 5324661 |
| CAS DataBase Reference | 333338-18-4(CAS DataBase Reference) |
Safety information for 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE
| Signal word | Warning |
| Pictogram(s) |
 Exclamation Mark Irritant GHS07 |
| GHS Hazard Statements |
H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation |
| Precautionary Statement Codes |
P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P271:Use only outdoors or in a well-ventilated area. P280:Wear protective gloves/protective clothing/eye protection/face protection. |
Computed Descriptors for 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE
4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE manufacturer
Innovative
Mumbai
product: P-Nitrophenylphosphate Disodium Salt Hexahydrate (PNPP) (CAS Number: 333338-18-4)
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