(4-Methylumbelliferyl)-N-acetyl-α-D-neuraminic acid sodium salt

Description

4-Methylumbelliferyl-N-acetyl-α-D-neuraminic acid is a fluorogenic substrate of neuraminidases. 4-Methylumbelliferyl-N-acetyl-α-D-neuraminic acid is cleaved by neuraminidases to release the fluorescence moiety 4-methylumbelliferyl (4-MU). 4-MU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high (7.12-10.3) pH, respectively, and an emission maximum ranging from 445 to 455 nm, increasing as pH decreases. 4-Methylumbelliferyl-N-acetyl-α-D-neuraminic acid has been used to quantify neuraminidase activity in mammalian cells and clinical isolates of influenza virus.

Chemical properties

Off-White to Yellow Solid

The Uses of (4-Methylumbelliferyl)-N-acetyl-α-D-neuraminic acid sodium salt

Used as a substrate in a fluorometric assay of neuraminidase.

The Uses of (4-Methylumbelliferyl)-N-acetyl-α-D-neuraminic acid sodium salt

(4-Methylumbelliferyl)-N-acetyl-α-D-neuraminic acid sodium salt has been used for fluorometric assay of neuraminidase as well as fluorescent staining of sialidases in PAGE.

What are the applications of Application

2′-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid sodium salt is a substrate for fluorometric assay of neuraminidase

What are the applications of Application

Neu5Ac-α-4MU is used as a substrate in a fluorometric assay of neuraminidase

Biochem/physiol Actions

Substrate for fluorometric assay of neuraminidase. Used for fluorescent staining of sialidases in PAGE.