3,3`-Dithiobispropanoic acid bis(N-hydroxysucciniMde ester)

Synonym(s):Di(N-succinimidyl) 3,3′-dithiodipropionate;Dithiobis(succinimidyl propionate);DTSP;Lomant’s reagent

CAS NO.：57757-57-0
Empirical Formula: C14H16N2O8S2
Molecular Weight: 404.42
MDL number: MFCD00042045
EINECS: 260-931-5
SAFETY DATA SHEET (SDS)
Update Date: 2025-07-24 18:13:54

3,3`-Dithiobispropanoic acid bis(N-hydroxysucciniMde ester) Structural

What is 3,3`-Dithiobispropanoic acid bis(N-hydroxysucciniMde ester)?

Description

DSP Crosslinker is a cleavable crosslinker. It contains two terminal NHS ester moieties which can react with primary amines. The disulfide bonds can be cleaved via a reduction reaction using Dithiothreitol (DTT) reagent.

Chemical properties

Off-White Solid

The Uses of 3,3`-Dithiobispropanoic acid bis(N-hydroxysucciniMde ester)

3,3`-Dithiobispropanoic acid bis(N-hydroxysucciniMde ester) can be used in the crosslinking of intracellular proteins prior to cell lysis.

The Uses of 3,3`-Dithiobispropanoic acid bis(N-hydroxysucciniMde ester)

3,3`-Dithiobispropanoic acid bis(N-hydroxysucciniMde ester) can be used as amine reactive homobifunctional cross-linking reagent, cleavable under mild conditions with hydroxylamine (pH 8.5, 3-6 h, 37oC)

The Uses of 3,3`-Dithiobispropanoic acid bis(N-hydroxysucciniMde ester)

3,3′-Dithiodipropionic acid di(N-hydroxysuccinimide ester) (DSP) has been used as a protein cross-linker.

What are the applications of Application

3,3′-Dithiodipropionic acid di(N-hydroxysuccinimide ester) is a cleavable disulfide linkage-containing homobifunctional cross-linking reagent

General Description

3,3′-Dithiodipropionic acid di(N-hydroxysuccinimide ester) (DSP) is a homobifunctional cross-linking reagent containing a cleavable disulfide linkage. It is typically coupled to molecules containing primary amines by amide bonds buffered at pH 7.5 (6.5-8.5).

Properties of 3,3`-Dithiobispropanoic acid bis(N-hydroxysucciniMde ester)

Melting point:	128-133 °C
Boiling point:	560.1±60.0 °C(Predicted)
Density	1.57±0.1 g/cm3(Predicted)
storage temp.	-20°C
solubility	chloroform: 50 mg/mL
form	powder
color	Off-White
BRN	1518074
CAS DataBase Reference	57757-57-0(CAS DataBase Reference)

Safety information for 3,3`-Dithiobispropanoic acid bis(N-hydroxysucciniMde ester)

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes	P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P264:Wash hands thoroughly after handling. P264:Wash skin thouroughly after handling. P271:Use only outdoors or in a well-ventilated area. P280:Wear protective gloves/protective clothing/eye protection/face protection. P302+P352:IF ON SKIN: wash with plenty of soap and water. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.