Tetrazine-PEG5-NHS ester

Synonym(s):Tz-PEG5-NHS

CAS NO.：1682653-80-0
Empirical Formula: C27H36N6O10
Molecular Weight: 604.61
MDL number: MFCD28505547
Update Date: 2026-01-13 11:26:06

What is Tetrazine-PEG5-NHS ester?

Description

Tetrazine-PEG5-NHS Ester is a PEG linker containing a tetrazine group and an NHS ester which is very reactive with amine(NH2)-containing molecule. Methyltetrazine enable fast click reaction with TCO (trans-cycloctene).

The Uses of Tetrazine-PEG5-NHS ester

Tetrazine-PEG5-NHS ester (Tz-PEG₅-NHS) can be used in click chemistry applications such as the preparation of chitosan-polyacrylamide microspheres utilizing selective tetrazine-trans-cyclooctene (Tz-TCO) ligation and the construction of copper-64 radiolabeled antibody by the click reaction.

General Description

Tetrazine-PEG5-NHS ester has an N-hydroxysuccinimide (NHS) ester that can be reacted with primary amines and tetrazine, which is reactive to trans-cyclooctenes. Hydrogen substituted tetrazines demonstrate exceptionally fast kinetics, generally at least 10-fold faster compared to methyl substituted tetrazines. The aqueous solubility of this reagent is substantially enhanced by a hydrophilic polyethylene glycol (PEG) spacer arm.

Properties of Tetrazine-PEG5-NHS ester

Density	1.34±0.1 g/cm3(Predicted)
storage temp.	-20°C
form	powder or crystals
pka	14.28±0.46(Predicted)
color	Purple to purplish red

Safety information for Tetrazine-PEG5-NHS ester

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H302:Acute toxicity,oral H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes	P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P280:Wear protective gloves/protective clothing/eye protection/face protection. P301+P312:IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell. P302+P352:IF ON SKIN: wash with plenty of soap and water. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.