SKOV3 Cells GFP-STAT3

The Uses of SKOV3 Cells GFP-STAT3

This product is a human SKOV3 cell line in which the genomic STAT3 gene has been endogenously tagged with a Green Fluorescent Protein (GFP) gene using CompoZr^? zinc finger nuclease technology. Integration resulted in endogenous expression of the fusion protein in which GFP is attached to the N-terminus of STAT3. Fluorescence imaging shows GFP-STAT3 expression throughout the cell. Upon activation with the ligand IL-6, the cell line shows redistribution of STAT3 from the cytoplasm to the nucleus, making it useful for high content screening of compounds that modulate STAT3 activity. This stable cell line was expanded from a single clone. The target′s gene regulation and corresponding protein function are preserved in contrast to cell lines with overexpression via an exogenous promoter.

To learn more, please visit the Cellular Reporter Cell Line webpage

General Description

SKOV3 GFP-STAT3 are ovarian adenocarcinoma cells from a human 64 year old caucasion female having a ZFN modification creating a GFP-STAT3 transgene expressed from the endogenous STAT3 gene locus.

This cell line was derived from ATCC Catalog No. HTB-77.