MUNG BEAN NUCLEASE

The Uses of MUNG BEAN NUCLEASE

The enzyme has an optimal temperature of approximately 70 °C, but for a long incubation, a temperature below 60 °C is more suitable. It is stable in the pH range of 5 - 8.

Definition

An enzyme which digests only single-stranded nucleic acids.

General Description

Nuclease P1 is one of the most commonly known single-strand specific nucleases in molecular biology; Nuclease P1 is a single stranded specific endoduclease (of ssDNA or ssRNA). Nuclease P1 can also cleave single-stranded regions in double-stranded nucleic acids.
Nuclease P1 from Penicillium citrinum is a zinc-dependent endonuclease that exhibits increased activity in the presence of low concentrations of urea. Nuclease P1 selective activity has found useful applications in studies on nucleic acid structure.

Biochem/physiol Actions

Catalyzes the nonspecific endonucleolytic cleavage of single stranded DNA and RNA to yield nucleoside 5′-phosphates and 5′-phosphooligonucleotides. It does not appreciably degrade double-stranded nucleic acids, especially in the presence of more than 400 mM sodium chloride at pH 6.0.